Order 3, Get Your 4th Free!* | Learn More >>
45115
CellSimple™ Annexin V-FITC Early Apoptosis Detection Kit
Cellular Assay Kits
Assay Kit

CellSimple™ Annexin V-FITC Early Apoptosis Detection Kit #45115

Citations (0)

We recommend the following alternatives

# Product Name Application Reactivity
  • IF
  • F
No image available
To Purchase # 45115

Product Description

The CellSimple™ Annexin V-FITC Early Apoptosis Detection Kit is designed for use with the CellSimple™ Cell Analyzer. It allows researchers to identify early apoptotic cells within a cell population. Annexin V-FITC conjugated protein binds to cell surfaces expressing phosphatidylserine, an early apotosis marker. Necrotic cells can be identified by staining with propidium iodide (PI), a non-cell-permeable DNA dye. Cells stained with both PI and annexin V-FITC are indicative of later stage apoptosis and early necrosis. This kit can be used together with the Apoptosis (Annexin V-FITC, PI) App on the CellSimple™ Cell Analyzer. This kit provides enough reagent to perform 100 assays, based on a 250 μl assay volume. The CellSimple™ Cell Analyzer comes complete with a pack of 25 cassettes (50 tests), a USB cord and power adapter, and user documentation.  

The CellSimple™ Cell Analyzer is currently only available in the US.

Specificity / Sensitivity

Species predicted to react based on 100% sequence homology:

All Species Expected

Background

The CellSimple™ Cell Analyzer is a benchtop instrument that utilizes a disposable thin-film cassette and a combination of a 488 nm laser, two photomultiplier tubes (525/45 nm and 561 nm LP filters), Coulter Principle-based cell measurements, and on-board software to provide easy-to-run applications and data analysis. Data acquisition occurs within approximately 10 seconds per test. The instrument relies on disposable cassettes for sample handling, which alleviates the need for flow cell cleaning and fluidics maintenance and the instrument is small enough to be portable between the lab bench and the hood. Applications include quantitative assessments of cell viability, apoptosis, other labeled antibody markers and single and multiplexed bead-based assays for protein and cellular analysis.

Annexin V belongs to the annexin family of proteins consisting of over 160 members. All annexin family members share the same charactersistic of Ca2+-dependent binding to negatively charged phospholipid surfaces (1). During early apoptosis, phosphatidylserine (PS) is translocated from the cytosolic side of the plasma membrane to the cellular surface. This translocation exposes PS to the extracellular environment with the plasma membrane left intact (2). Annexin V protein demonstrates high affinity, specificity, and sensitivity for PS and can be used as a marker of early apoptosis. In order to rule out "leaky" necrotic cells, annexin V must be used in tandem with reagents that determine the integrity of the cell membrane, such as propidium iodide (2).

  1. Boersma, H.H. et al. (2005) J Nucl Med 46, 2035-50.
  2. Vermes, I. et al. (1995) J Immunol Methods 184, 39-51.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
CellSimple is a trademark of Cell Signaling Technology, Inc.