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15472
CellSimple™ Epithelial to Mesenchymal Transition Antibody Assay Kit
Cellular Assay Kits
Assay Kit

CellSimple™ Epithelial to Mesenchymal Transition Antibody Assay Kit #15472

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Product Description

CellSimple™ Epithelial to Mesenchymal Transition Antibody Assay Kit is a fluorescent assay designed for use with the CellSimple™ Cell Analyzer. The kit includes E-Cadherin (24E10) Rabbit mAb (Alexa Fluor® 488) #3199 and Vimentin (D21H3) XP® Rabbit mAb (PE Conjugate) #12020, that can differentiate between epithelial and mesenchymal cell origins, respectively. Thus mean fluorescence intensity (MFI) in the green (525/45 nm) and red (561 nm LP) channels can be used as an indicator for cells undergoing epithelial-mesenchymal transition (EMT).

Specificity / Sensitivity

Antibodies provided in the CellSimple™ Epithelial to Mesenchymal Transition Antibody Assay Kit detect endogenous levels of their respective target.

Background

The CellSimple™ Cell Analyzer is a benchtop instrument that utilizes a disposable thin-film cassette and a combination of a 488 nm laser, two photomultiplier tubes (525/45 nm and 561 nm LP filters), Coulter Principle-based cell measurements, and on-board software to provide easy-to-run applications and data analysis. Data acquisition occurs within approximately 10 seconds per test. The instrument relies on disposable cassettes for sample handling, which alleviates the need for flow cell cleaning and fluidics maintenance and the instrument is small enough to be portable between the lab bench and the hood. Applications include quantitative assessments of cell viability, apoptosis, other labeled antibody markers and single and multiplexed bead-based assays for protein and cellular analysis.

Epithelial-mesenchymal transition (EMT) is an essential process during development whereby epithelial cells aquire mesenchymal, fribroblast-like properties and display reduced intracellular adhesion and increased motility. This is a critical feature of normal embryonic development, which is also utilized by malignant epithelial tumors to spread beyond their origin (1-3). This tightly regulated process is associated with a number of cellular and molecular events. EMT depends on a reduction in expression of cell adhesion molecules. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (4). E-cadherin is considered to be an active suppressor of invasion and growth in many epithelial cancers (4-6). Recent studies indicate that cancer cells have upregulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch" and downregulation of E-cadherin is one of the hallmarks of EMT (1). Cells undergoing EMT may also continue to express epithelial markers and show epithelial morphology while exhibiting increased expression of mesenchymal markers such as vimentin (7).

  1. Aigner, K. et al. (2007) Oncogene 26, 6979-88.
  2. Peinado, H. et al. (2007) Nat Rev Cancer 7, 415-28.
  3. Moreno-Bueno, G. et al. (2008) Oncogene 27, 6958-69.
  4. Wheelock, M.J. and Johnson, K.R. (2003) Annu Rev Cell Dev Biol 19, 207-35.
  5. Christofori, G. (2003) EMBO J 22, 2318-23.
  6. Hazan, R.B. et al. (2004) Ann N Y Acad Sci 1014, 155-63.
  7. Kalluri, R. and Weinberg, R.A. (2009) J Clin Invest 119, 1420-8.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
CellSimple is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
The Alexa Fluor dye conjugates in this product are sold under license from Life Technologies Corporation, for research use only excluding use in combination with DNA microarrays and high content screening (HCS).