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29964
CellSimple™ Performance beads
Cellular Assay Kits
Miscellaneous

CellSimple™ Performance beads #29964

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CellSimple™ Performance beads: Image 1

CellSimple™ Performance Beads were analyzed using the Open Flow Cytometry Application detecting in both green (525/45nm, left panel) and red (561nm LP, right panel) channels. Non-fluorescent and fluorescent beads were gated according to Median Fluorescence Intensity (MFI) and diameter (μm). Results are shown in the black rectangular boxes below the dot plots. Instrument screen shots are shown.

Product Usage Information

Prior to use, allow the beads to equilibrate to room temperature for at least 15 minutes. From the CellSimple™ Cell Analyzer main screen, choose the Open Flow Cytometry application and select both detection channels PMT1 (525/45 nm) and PMT2 (561 nm LP). The fluorescent gain setting should be set to “default”. Immediately prior to loading the Performance Beads, vortex them on high for 15 seconds then pipette 75 μl into the cassette and analyze. Do not dilute the aqueous solution. Gate the bead populations according to MFI and diameter (μm). Please see the CellSimple™ User Guide for more details about using the Open Flow Cytometry Application.

Storage

Store at 4° C and protect from light. Do not freeze as this will compromise bead performance. The Performance Beads are stable for at least 12 months when stored at the recommended conditions.

Product Description

CellSimple™ Performance Beads are available as a quality control reagent for use with the CellSimple™ Cell Analyzer. The reagent contains a mixture of four beads: smaller red and larger green fluorescent beads, each with size-matched non-fluorescent beads. The four-bead mixture is run simultaneously and gated to determine fluorescence intensity, diameter and total count. The Performance Beads can be used on a regular basis to check instrument performance and reproducibility. Example data are shown in the attached figures.

Background

The CellSimple™ Cell Analyzer is a benchtop instrument that utilizes a disposable thin-film cassette and a combination of a 488 nm laser, two photomultiplier tubes (525/45 nm and 561 nm LP filters), Coulter Principle-based cell measurements, and on-board software to provide easy-to-run applications and data analysis. Data acquisition occurs within approximately 10 seconds per test. The instrument relies on disposable cassettes for sample handling, which alleviates the need for flow cell cleaning and fluidics maintenance and the instrument is small enough to be portable between the lab bench and the hood. Applications include quantitative assessments of cell viability, apoptosis, other labeled antibody markers and single and multiplexed bead-based assays for protein and cellular analysis.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
CellSimple is a trademark of Cell Signaling Technology, Inc.
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