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24417
CellSimple™ Propidium Iodide (PI)/RNase Staining Solution Kit
Cellular Assay Kits
Assay Kit

CellSimple™ Propidium Iodide (PI)/RNase Staining Solution Kit #24417

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Product Description

CellSimple™ Propidium Iodide (PI)/RNase Staining Solution Kit is designed for use with the CellSimple™ Cell Analyzer. It detects cellular DNA content in both living and fixed cells. The median fluorescent intensity (MFI) of the red emission (PMT1) can be used as an indicator for cellular DNA content. This kit can be used together with Cell Cycle App on the CellSimple™ Cell Analyzer for cell cycle progression analysis. At the recommended reagent concentrations and volumes, one kit will provide for 100 assays. The CellSimple™ Cell Analyzer comes complete with a pack of 25 cassettes (50 tests), a USB cord and power adapter, and user documentation.

The CellSimple™ Cell Analyzer is currently only available in the US.

Specificity / Sensitivity

Species predicted to react based on 100% sequence homology:

All Species Expected

Background

The CellSimple™ Cell Analyzer is a benchtop instrument that utilizes a disposable thin-film cassette and a combination of a 488 nm laser, two photomultiplier tubes (525/45 nm and 561 nm LP filters), Coulter Principle-based cell measurements, and on-board software to provide easy-to-run applications and data analysis. Data acquisition occurs within approximately 10 seconds per test. The instrument relies on disposable cassettes for sample handling, which alleviates the need for flow cell cleaning and fluidics maintenance and the instrument is small enough to be portable between the lab bench and the hood. Applications include quantitative assessments of cell viability, apoptosis, other labeled antibody markers and single and multiplexed bead-based assays for protein and cellular analysis.

Propidium Iodide (PI) is a fluorescent dye which intercalates between bases and stains both DNA and RNA. Specific DNA staining is achieved by enzymatic removal of RNA with a ribonuclease (RNase). PI/RNase is commonly used as a nuclear stain in fluorescent microscopy and as a DNA content determinant in cell cycle analyses by flow cytometry. Cells in G2 and M phases of the cell cycle contain twice the DNA content compared to those in G0 and G1 phases. DNA content during S phase lies between these extremes. PI/RNase Staining Solution has excitation and emission maxima of 535 and 617 nm, respectively (orange to red range of the spectrum).

  1. Suzuki, T. et al. (1997) J Histochem Cytochem 45, 49-53.
  2. Jones, K.H. and Kniss, D.A. (1987) J Histochem Cytochem 35, 123-5.
  3. Deitch, A.D. et al. (1982) J Histochem Cytochem 30, 967-72.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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