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CellSimple™ Stem Cell Pluripotency Antibody Assay Kit
Assay Kit

CellSimple™ Stem Cell Pluripotency Antibody Assay Kit #62734

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CellSimple™ Stem Cell Pluripotency Antibody Assay Kit: Image 1
CellSimple™ cell-based analysis of fixed and permeabilized HeLa (left) and NTERA-2 (right) using CellSimple™ Stem Cell Pluripotency Antibody Assay Kit. Data was collected in both green (525/45 nm) and red (561 nm LP) channels and analyzed using the Open Flow Application. Dot plots represent mean fluorescence intensity (MFI) of Oct-4A (C30A3) Rabbit mAb (Alexa Fluor® 488 Conjugate) #5177 in the green channel (x-axis) and Nanog (D73G4) XP® Rabbit mAb (PE Conjugate) #14955 in the red channel (y-axis). Instrument screen shots are shown.
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Product Description

CellSimple™ Stem Cell Pluripotency Antibody Assay Kit is designed for use with the CellSimple™ Cell Analyzer. The kit allows for the quick and easy measurement of Nanog and Oct-4A, two key proteins that are specifically expressed in human pluripotent cells. The kit includes Nanog (D73G4) XP® Rabbit mAb (PE Conjugate) #14955 and Oct-4A (C30A3) Rabbit mAb (Alexa Fluor® 488 Conjugate) #5177.

Specificity / Sensitivity

Antibodies provided in the CellSimple™ Stem Cell Pluripotency Antibody Assay Kit detect endogenous levels of their respective target.

Species Reactivity:



The CellSimple™ Cell Analyzer is a benchtop instrument that utilizes a disposable thin-film cassette and a combination of a 488 nm laser, two photomultiplier tubes (525/45 nm and 561 nm LP filters), Coulter Principle-based cell measurements, and on-board software to provide easy-to-run applications and data analysis. Data acquisition occurs within approximately 10 seconds per test. The instrument relies on disposable cassettes for sample handling, which alleviates the need for flow cell cleaning and fluidics maintenance and the instrument is small enough to be portable between the lab bench and the hood. Applications include quantitative assessments of cell viability, apoptosis, other labeled antibody markers and single and multiplexed bead-based assays for protein and cellular analysis. Pluripotency is the ability of a cell to differentiate into cell types of the three germ layers, the endoderm, ectoderm, and mesoderm. It is a property shared by embryonic stem cells, embryonic carcinoma and induced pluripotent cells. Oct-4 and Nanog together with Sox2 are key transcriptional regulators that are highly expressed in pluripotent cells (1). Together they form a transcriptional network that maintains cells in a pluripotent state (2,3). Overexpression of Oct-4 and Sox2 along with Klf4 and c- Myc can induce pluripotency in both mouse and human somatic cells, highlighting their roles as key regulators of the transcriptional network necessary for renewal and pluripotency (4-5). Upon differentiation of pluripotent cultures, expression of Oct-4 and Nanog is induced.
  1. Pesce, M. and Schöler, H.R. (2001) Stem Cells 19, 271-8.
  2. Pan, G. and Thomson, J.A. (2007) Cell Res 17, 42-9.
  3. Takahashi, K. and Yamanaka, S. (2006) Cell 126, 663-76.
  4. Okita, K. et al. (2007) Nature 448, 313-7.
  5. Yu, J. et al. (2007) Science 318, 1917-20.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
CellSimple is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
The Alexa Fluor dye conjugates in this product are sold under license from Life Technologies Corporation, for research use only excluding use in combination with DNA microarrays and high content screening (HCS).