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7802
HTScan® Csk Kinase Assay Kit
Cellular Assay Kits
Assay Kit

HTScan® Csk Kinase Assay Kit #7802

Citations (0)
HTScan® Csk Kinase Assay Kit: Image 1
Western blot analysis of extracts from Jurkat cells treated with 1 mM pervanadate for 30 minutes prior to lysis, using P-Tyr-100 Phospho-Tyrosine Mouse mAb. Proteins were separated by 2-D electrophoresis prior to blotting.
HTScan® Csk Kinase Assay Kit: Image 2
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-Tyrosine Mouse mAb (P-Tyr-100).
HTScan® Csk Kinase Assay Kit: Image 3
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-Tyrosine Mouse mAb (P-Tyr-100).
HTScan® Csk Kinase Assay Kit: Image 4
Immunohistochemical analysis of paraffin-embedded NCI-H1650 xenograft untreated (left) or lambda-phosphatase-treated (right), using Phospho-Tyrosine Mouse mAb (P-Tyr-100).
HTScan® Csk Kinase Assay Kit: Image 5
Immunohistochemical analysis of paraffin-embedded human B-cell non-Hodgkin lymphoma, untreated (left) or lambda phosphatase treated (right), using Phospho-Tyrosine Mouse mAb (P-Tyr-100).
HTScan® Csk Kinase Assay Kit: Image 6
Immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using Phospho-Tyrosine Mouse mAb (P-Tyr-100).
HTScan® Csk Kinase Assay Kit: Image 7
Immunohistochemical analysis using Phospho-Tyrosine Mouse mAb (P-Tyr-100) on SignalSlide™ Phospho-EGF Receptor IHC Controls #8102 (paraffin-embedded KYSE450 cell pellets, untreated (left) or EGF-treated (right)).
HTScan® Csk Kinase Assay Kit: Image 8
Immunohistochemical analysis of paraffin-embedded human soft tissue squamous cell carcinoma using Phospho-Tyrosine Mouse mAb (P-Tyr-100).
HTScan® Csk Kinase Assay Kit: Image 9
Immunohistochemical analysis of paraffin-embedded normal human kidney using Phospho-Tyrosine Mouse mAb (P-Tyr-100).
HTScan® Csk Kinase Assay Kit: Image 10
Immunohistochemical analysis of paraffin-embedded normal human lung using Phospho-Tyrosine Mouse mAb (P-Tyr-100).
HTScan® Csk Kinase Assay Kit: Image 11
Immunohistochemical analysis of paraffin-embedded NCI-H1650 xenograft using Phospho-Tyrosine Mouse mAb (P-Tyr-100).
HTScan® Csk Kinase Assay Kit: Image 12
Confocal immunofluorescent analysis of paraffin-embedded human lung adenocarcinoma using Phospho-Tyrosine Mouse mAb (P-Tyr-100) (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).
HTScan® Csk Kinase Assay Kit: Image 13
Immunofluorescent analysis of Swiss NIH/3T3 cells, serum-starved and stimulated with lysophosphatidic acid (LPA) (10 µM for 10 minutes) and fixed with PFA, using Phospho-Tyrosine Mouse mAb (P-Tyr-100) (red) and phalloidin for F-actin (green) LPA causes heavy tyrosine phosphorylation of proteins in focal adhesions, present at the tips of actin stress fibers. (Provided by Dr. Harry Mellor, University of Bristol.)
HTScan® Csk Kinase Assay Kit: Image 14
Confocal immunofluorescence analysis of HeLa cells either stimulated with 20% serum (left) or untreated (right), using Phospho-Tyrosine Mouse mAb (P-Tyr-100) (red). Actin filaments have been labeled with fluorescein phalloidin. Blue pseudocolor = DRAQ5® (fluorescent DNA dye).
HTScan® Csk Kinase Assay Kit: Image 15
Flow cytometric analysis of K562 cells, untreated (green) or Gleevec®- treated (blue), using Phospho-Tyrosine Mouse mAb (P-Tyr-100) compared to a nonspecific negative control antibody (red).
Inquiry Info.# 7802

Product Description

The kit provides a means of performing kinase activity assays with recombinant human Csk kinase. It includes active Csk kinase (supplied as a GST fusion protein), a biotinylated peptide substrate and a phospho-Tyrosine monoclonal antibody for detection of the phosphorylated form of the substrate peptide.
MW (kDa) biotin peptide: 2290 Daltons, GST-Csk: 80
Molecular Formula Peptide substrate, Biotin-peptide: 2,290 Daltons, GST-Csk Kinase: 80 kDa.

Background

Carboxy-terminal Src kinase (Csk) is a ubiquitously expressed nonreceptor tyrosine kinase that negatively regulates the Src family kinases (SFKs) by phosphorylation of the SFK carboxy-terminal tyrosine (1,2). Phosphorylated carboxy-terminal tyrosine binds to the SH2 domain of SFK intramolecularly and leads to folding and inactivation of the SFK (3). This Csk-catalyzed SFK tyrosine phosphorylation is highly specific and exclusive. The SFK carboxy-terminal tyrosine is the only known physiological substrate of Csk (4). Csk consists of an SH2, an SH3, and a kinase domain. There is evidence that the SH2 and SH3 domains are essential for the regulation of SFK, and Csk can be recruited to the membrane where SFKs are in an active state. This process is mediated by a Csk-binding protein (Cbp, also called PAG), which binds tightly to the SH2 domain of Csk (5). Activation of SFK by extracellular stimuli leads to the tyrosine phosphorylation of Cbp, generating docking sites for Csk. The recruitment of Csk forms a feedback mechanism for termination of SFK function (6).
  1. Nada, S. et al. (1991) Nature 351, 69-72.
  2. Nada, S. et al. (1993) Cell 73, 1125-35.
  3. Lee, S. et al. (2003) Proc Natl Acad Sci U S A 100, 14707-12.
  4. Imamoto, A. and Soriano, P. (1993) Cell 73, 1117-24.
  5. Kawabuchi, M. et al. (2000) Nature 404, 999-1003.
  6. Matsuoka, H. et al. (2004) J Biol Chem 279, 5975-83.

Pathways & Proteins

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