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HTScan® MAPKAPK-5 Kinase Assay Kit
Cellular Assay Kits
Assay Kit

HTScan® MAPKAPK-5 Kinase Assay Kit #7660

Citations (0)
HTScan® MAPKAPK-5 Kinase Assay Kit: Image 1
Western blot analysis of extracts from PC12 cells, untreated or forskolin-treated (30 µM), using Phospho-Tyrosine Hydroxylase (Ser40) Antibody (upper) or Tyrosine Hydroxylase Antibody #2792 (lower).
HTScan® MAPKAPK-5 Kinase Assay Kit: Image 2
Confocal immunofluorescent images of PC-12 cells, untreated (A,C) and calf intestinal phosphatase treated (B), labeled with Phospho-Tyrosine Hydroxylase (Ser40) Antibody #2791 (green, top) and Tyrosine Hydroxylase Antibody #2792 (green, bottom) compared to an isotype control (D). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Inquiry Info.# 7660

Product Description

The kit provides a means of performing kinase activity assays with recombinant human MAPKAPK-5 kinase. It includes active MAPKAPK-5 kinase (supplied as a GST fusion protein), a biotinylated peptide substrate and a phospho-serine/threonine antibody for detection of the phosphorylated form of the substrate peptide.
MW (kDa) Peptide substrate, Biotin- Tyrosine Hydroxylase (Ser40): 2,326 Daltons. GST-MAPKAPK-5 Kinase: 84
Molecular Formula Peptide substrate, Biotin- Tyrosine Hydroxylase (Ser40): 2,326 Daltons. GST-MAPKAPK-5 Kinase: 84 kDa.


MAPKAPK-5 belongs to the mitogen-activated protein kinase (MAPK) activated protein kinases (MK) subfamily that includes MAPKAPK-2/MK2 and MK3/3pK. The MK subfamily is part of a family of protein kinase subfamilies downstream of MAPK pathways and includes ribosomal S6 kinase (RSKs), mitogen and stress activated kinases (MSKs) and MAPK-interacting kinases (MNKs). All MKs are activated by MAPK pathways and mediate important processes (e.g. gene expression, cell cycle progression) and have been implicated in inflammation and cancer (1,2). MAPKAPK-5 shows binding to and activation by p38 MAPK and extracellular-regulated kinases (Erk) (3,4). MAPKAPK-5 was shown to be activated by Erk3 and act as a chaperone to Erk3 (5,6). While overexpressed MAPKAPK-5 shares similar substrates with MAPKAPK-2, such as HSP27 and glycogen synthase, recent work with MAPKAPK-5 knock-out mice indicates distinct substrates and functional properties (7).
  1. Roux, P.P. and Blenis, J. (2004) Microbio.l Mol. Biol. Rev. 68, 320-344.
  2. Gaestel, M. (2006) Nat. Rev. Mol. Cell Biol. 7, 120-130.
  3. New, L. et al. (1998) EMBO J. 17, 3372-3384.
  4. Ni, H. et al. (1998) Biochem. Biophys. Res. Commun. 243, 492-496.
  5. Schumacher, S. et al. (2004) EMBO J. 23, 4770-4779.
  6. Seternes, O.M. et al. (2004) EMBO J. 23, 4780-4791.
  7. Shi, Y. et al. (2003) Mol. Cell Biol. 23, 7732-7741.

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