20% off purchase of 2 or more Monoclonal Antibodies* | Learn More >>
7582
HTScan® PKCβ I Kinase Assay Kit

HTScan® PKCβ I Kinase Assay Kit #7582

This product is discontinued

Kinase Assay - Radiometric Image 1

Figure 1. PKCbeta I kinase activity was measured in a radiometric assay using the following reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, 1 µM ATP, 2.5 µg/50 µl PEG20,000, Substrate: Histone H1, 1 µg/50 µl and Recombinant PKCbeta I: 50 ng/50 µl.

Learn more about how we get our images
Kinase Assay - DELFIA Image 2

Figure 3. Dose dependence curve of PKCbeta I kinase activity: DELFIA® data generated using Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb #9624 to detect phosphorylation of substrate peptide (#1331) by PKCbeta I kinase. In a 50 µl reaction, increasing amounts of PKCbeta I and 1.5 µM substrate peptide were used per reaction at room temperature for 15 minutes. (DELFIA® is a registered trademark of PerkinElmer, Inc.)

Learn more about how we get our images
Kinase Assay - DELFIA Image 3

Figure 5. Staurosporine inhibition of PKCbeta I kinase activity: DELFIA® data generated using Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb #9624 to detect phosphorylation of PKCbeta I substrate peptide (#1331) by PKCbeta I kinase. In a 50 µl reaction, 10 ng PKCbeta I, 1.5 µM substrate peptide, 20 µM ATP and increasing amounts of staurosporine were used per reaction at room temperature for 15 minutes. (DELFIA® is a registered trademark of PerkinElmer, Inc.)

Learn more about how we get our images
Kinase Assay - DELFIA Image 4

Figure 4. Peptide concentration dependence of PKCbeta I kinase activity: DELFIA® data generated using Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb #9624 to detect phosphorylation of substrate peptide (#1331) by PKCbeta I kinase. In a 50 µl reaction, 10 ng of PKCbeta I and increasing concentrations of substrate peptide were used per reaction at room temperature for 15 minutes. (DELFIA® is a registered trademark of PerkinElmer, Inc.)

Learn more about how we get our images
Kinase Assay - DELFIA Image 5

Figure 2. Time course of PKCbeta I kinase activity: DELFIA® data generated using Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb #9624 to detect phosphorylation of PKCbeta1 substrate peptide (#1331) by PKCbeta I kinase. In a 50 µl reaction, 10 ng PKCbeta I and 1.5 uM substrate peptide were used per reaction. (DELFIA® is a registered trademark of PerkinElmer, Inc.)

Learn more about how we get our images
Product Includes Quantity (with Count) Solution Color
ATP (10 mM) 9804 1 x 1 ml
Kinase Buffer (10X) 9802 1 x 15 ml

The kit provides a means of performing kinase activity assays with recombinant human PKCbeta I kinase. It includes active PKCbeta I kinase (supplied as a GST fusion protein), a biotinylated peptide substrate and a phospho-serine/threonine antibody for detection of the phosphorylated form of the substrate peptide.

Molecular Formula:

Peptide substrate, Biotin-CREB (Ser133): 2,326 Daltons. GST-PKCbeta1 Kinase: 104 kDa.

Peptide Core Sequence:

RRPS*YRK

Activation of protein kinase C (PKC) is one of the earliest events in a cascade that controls a variety of cellular responses, including secretion, gene expression, proliferation, and muscle contraction (1,2). PKC isoforms belong to three groups based on calcium dependency and activators. Classical PKCs are calcium-dependent via their C2 domains and are activated by phosphatidylserine (PS), diacylglycerol (DAG), and phorbol esters (TPA, PMA) through their cysteine-rich C1 domains. Both novel and atypical PKCs are calcium-independent, but only novel PKCs are activated by PS, DAG, and phorbol esters (3-5). Members of these three PKC groups contain a pseudo-substrate or autoinhibitory domain that binds to substrate-binding sites in the catalytic domain to prevent activation in the absence of cofactors or activators. Control of PKC activity is regulated through three distinct phosphorylation events. Phosphorylation occurs in vivo at Thr500 in the activation loop, at Thr641 through autophosphorylation, and at the carboxy-terminal hydrophobic site Ser660 (2). Atypical PKC isoforms lack hydrophobic region phosphorylation, which correlates with the presence of glutamic acid rather than the serine or threonine residues found in more typical PKC isoforms. The enzyme PDK1 or a close relative is responsible for PKC activation. A recent addition to the PKC superfamily is PKCμ (PKD), which is regulated by DAG and TPA through its C1 domain. PKD is distinguished by the presence of a PH domain and by its unique substrate recognition and Golgi localization (6). PKC-related kinases (PRK) lack the C1 domain and do not respond to DAG or phorbol esters. Phosphatidylinositol lipids activate PRKs, and small Rho-family GTPases bind to the homology region 1 (HR1) to regulate PRK kinase activity (7).

Both PKCbeta I and PKCbeta II are formed from a single gene locus (PKCbeta) by alternative splicing of the carboxy-terminal exons. PKCbetas are the major PKC isoforms in a variety of tissues and function in various signaling pathways regulating proliferation, differentiation, metabolism and cell-type-specific functions (10). In colon cancer, PKCbeta II appears to be overexpressed early in the carcinogenic process while PKCbeta I expression decreases later in tumor development (11).

  1. Nishizuka, Y. (1984) Nature 308, 693-8.
  2. Keranen, L.M. et al. (1995) Curr Biol 5, 1394-403.
  3. Mellor, H. and Parker, P.J. (1998) Biochem J 332 ( Pt 2), 281-92.
  4. Ron, D. and Kazanietz, M.G. (1999) FASEB J 13, 1658-76.
  5. Moscat, J. and Diaz-Meco, M.T. (2000) EMBO Rep 1, 399-403.
  6. Baron, C.L. and Malhotra, V. (2002) Science 295, 325-8.
  7. Flynn, P. et al. (2000) J Biol Chem 275, 11064-70.
  8. Kawakami, T. et al. (2002) J. Biochem. (Tokyo) 132, 677-82.
  9. Mackay, H.J. and Twelves, C.J. (2003) Endocr. Relat. Cancer 10, 389-96.
Entrez-Gene Id
5579
Swiss-Prot Acc.
P05771
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
DELFIA is a registered trademark of PerkinElmer, Inc.

Upstream / Downstream

pathwayImage

Explore pathways related to this product.