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7785
HTScan® VEGF Receptor 1 Kinase Assay Kit
Cellular Assay Kits
Assay Kit

HTScan® VEGF Receptor 1 Kinase Assay Kit #7785

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other Image 1 - HTScan® VEGF Receptor 1 Kinase Assay Kit

Figure 3. Dose dependence curve of VEGFR-1 kinase activity: DELFIA® data generated using Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 to detect phosphorylation of substrate peptide (#1310) by VEGFR-1 kinase. In a 50 µl reaction, increasing amounts of VEGFR-1 and 1.5 µM substrate peptide were used per reaction at room temperature for 30 minutes. (DELFIA® is a registered trademark of PerkinElmer, Inc.)

other Image 2 - HTScan® VEGF Receptor 1 Kinase Assay Kit

Figure 5. Staurosporine inhibition of VEGFR-1 kinase activity: DELFIA® data generated using Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 to detect phosphorylation of VEGFR-1 substrate peptide (#1310) by VEGFR-1 kinase. In a 50 µl reaction, 50 ng VEGFR-1, 1.5 µM substrate peptide, 20 µM ATP and increasing amounts of staurosporine were used per reaction at room temperature for 30 minutes. (DELFIA® is a registered trademark of PerkinElmer, Inc.)

other Image 3 - HTScan® VEGF Receptor 1 Kinase Assay Kit

Figure 4. Peptide concentration dependence of VEGFR-1 kinase activity: DELFIA® data generated using Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 to detect phosphorylation of substrate peptide (#1310) by VEGFR-1 kinase. In a 50 µl reaction, 50 ng of VEGFR-1 and increasing concentrations of substrate peptide were used per reaction at room temperature for 30 minutes. (DELFIA® is a registered trademark of PerkinElmer, Inc.)

other Image 4 - HTScan® VEGF Receptor 1 Kinase Assay Kit

Figure 2. Time course of VEGFR-1 kinase activity: DELFIA® data generated using Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 to detect phosphorylation of VEGFR-1 substrate peptide (#1310) by VEGFR-1 kinase. In a 50 µl reaction, 50 ng VEGFR-1 and 1.5 µM substrate peptide were used per reaction. (DELFIA® is a registered trademark of PerkinElmer, Inc.)

other Image 5 - HTScan® VEGF Receptor 1 Kinase Assay Kit

Figure 1. VEGFR-1 kinase activity was measured in a radiometric assay using the following reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20,000, Substrate: PolyEY5 µg/50 µl, recombinant VEGFR-1: 100 ng/50 µl.

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Product Description

The kit provides a means of performing kinase activity assays with recombinant human VEGFR-1 kinase. It includes active VEGFR-1 kinase (supplied as a GST fusion protein), a biotinylated peptide substrate and a phospho-tyrosine antibody for detection of the phosphorylated form of the substrate peptide.

Molecular Formula

Peptide substrate, Biotin-Gastrin Precursor (Tlyr87): 2,853 Daltons. GST-VEGFR-1 Kinase: 89 kDa.

Background

The vascular endothelial growth factor (VEGF) receptor (VEGFR-1, Flt-1) is a 180 kDa receptor tyrosine kinase belonging to the VEGFR (Flt) family (1-3). The receptor is comprised of seven extracellular Ig-like domains, a single transmembrane region and cytoplasmic tail containing the active kinase domain (1,2). VEGFR-1 plays an important role in endothelial cell function and normal vascular development, as well as in hematopoietic function (2,3). VEGF-A is a high affinity ligand of VEGFR-1. VEGFR-1 also binds VEGF-B and PLGF (2). Ligand binding results in very little VEGFR-1 kinase activation, and VEGFR-1/VEGF-A binding negatively regulates VEGF function by diverting the growth factor from other functional VEGF receptors (3).

  1. Ferrara, N. et al. (2003) Nat Med 9, 669-76.
  2. Clauss, M. (2000) Semin Thromb Hemost 26, 561-9.
  3. Claesson-Welsh, L. (2003) Biochem Soc Trans 31, 20-4.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
DELFIA is a registered trademark of PerkinElmer, Inc.