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7791
HTScan® VEGF Receptor 3 Kinase Assay Kit
Cellular Assay Kits
Assay Kit

HTScan® VEGF Receptor 3 Kinase Assay Kit #7791

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other Image 1 - HTScan® VEGF Receptor 3 Kinase Assay Kit

Figure 3. Dose dependence curve of VEGFR-3 kinase activity: DELFIA® data generated using Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 to detect phosphorylation of substrate peptide (#1367) by VEGFR-3 kinase. In a 50 µl reaction, increasing amounts of VEGFR-3 and 1.5 µM substrate peptide were used per reaction at room temperature for 30 minutes. (DELFIA® is a registered trademark of PerkinElmer, Inc.)

other Image 2 - HTScan® VEGF Receptor 3 Kinase Assay Kit

Figure 5. Staurosporine inhibition of VEGFR-3 kinase activity: DELFIA® data generated using Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 to detect phosphorylation of VEGFR-3 substrate peptide (#1367) by VEGFR-3 kinase. In a 50 µl reaction, 100 ng VEGFR-3, 1.5 µM substrate peptide, 20 µM ATP and increasing amounts of staurosporine were used per reaction at room temperature for 30 minutes. (DELFIA® is a registered trademark of PerkinElmer, Inc.)

other Image 3 - HTScan® VEGF Receptor 3 Kinase Assay Kit

Figure 4. Peptide concentration dependence of VEGFR-3 kinase activity: DELFIA® data generated using Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 to detect phosphorylation of substrate peptide (#1367) by VEGFR-3 kinase. In a 50 µl reaction, 100 ng of VEGFR-3 and increasing concentrations of substrate peptide were used per reaction at room temperature for 30 minutes. (DELFIA® is a registered trademark of PerkinElmer, Inc.)

other Image 4 - HTScan® VEGF Receptor 3 Kinase Assay Kit

Figure 2. Time course of VEGFR-3 kinase activity: DELFIA® data generated using Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 to detect phosphorylation of VEGFR-3 substrate peptide (#1367) by VEGFR-3 kinase. In a 50 µl reaction, 100 ng VEGFR-3 and 1.5 µM substrate peptide were used per reaction. (DELFIA® is a registered trademark of PerkinElmer, Inc.)

other Image 5 - HTScan® VEGF Receptor 3 Kinase Assay Kit

Figure 1. VEGFR-3 kinase activity was measured in a radiometric assay using the following reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20,000, Substrate: PolyEY5 µg/50 µl, recombinant VEGFR-3: 100 ng/50 µl.

To Purchase # 7791

Product Description

The kit provides a means of performing kinase activity assays with recombinant human VEGFR-3 kinase. It includes active VEGFR-3 kinase (supplied as a GST fusion protein), a biotinylated peptide substrate and a phospho-tyrosine antibody for detection of the phosphorylated form of the substrate peptide.

Molecular Formula

Peptide substrate, Biotin-MET (Tyr1253) peptide: 2,200Daltons. GST-VEGFR-3 Kinase: 86 kDa.

Background

Vascular endothelial growth factor receptor 3 (VEGFR3) is a 195 kDa membrane receptor tyrosine kinase. VEGF receptors are characterized by the presence of seven extracellular immunoglobulin (Ig)-like domains followed by a membrane-spanning domain and a conserved intracellular tyrosine kinase domain (1). VEGF receptor 3 expression is largely restricted to adult lymphatic endothelium and is thought to control lymphangiogenesis (1,2). Binding of VEGF-C/VEGF-D to VEGFR3 results in transphosphorylation of tyrosine residues in its intracellular domain, recruitment of signaling molecules and activation of ERK1/2 and Akt signaling cascades (1,3).

  1. Robinson, C.J. and Stringer, S.E. (2001) J Cell Sci 114, 853-65.
  2. Valtola, R. et al. (1999) Am. J. Pathol. 154, 1381-1390.
  3. Saharinen, P. and Petrova, T.V. (2004) Ann. N.Y. Acad. Sci. 1014, 76-87.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
DELFIA is a registered trademark of PerkinElmer, Inc.