Rapid-Act T Cell Activation Kit (Human, Anti-CD3/CD28) (#88179) Datasheet Without Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Information

Storage

Store at 4ºC. All components in this kit are stable for 12 months when kept in the original format and stored at 4ºC. Once reconstituted, store the Anti-human CD3 and Anti-human CD28 at -80ºC. Aliquot to avoid multiple freeze/thaw cycles.

Specificity / Sensitivity

Species Reactivity:

Human

Product Description

The Rapid-Act T Cell Activation Kit (Human, Anti-CD3/CD28) provides the necessary reagents for easy, single-step induction of human T cell activation and proliferation. The included Synthetic Immune Synapse particles pre-cluster the CD3 and CD28 antibodies for thorough T cell activation, and are small enough to minimize cell clumping. Because the reagents are added to the cells in a single step, the kit enables accurate time course studies for short-term T cell stimulation. The kit can also be used for longer-term T cell proliferation and exhaustion assays. No modification or removal is necessary prior to subsequent analysis by flow cytometry.

Background

T cells are activated by signaling through ITAM (immunoreceptor tyrosine-based activation motif)-containing CD3 signaling chains that associate with the T cell receptor (1). Co-stimulation through CD28 on T cells provides an additional signal required for effective T cell activation (2). Antibody-based binding and aggregation of CD3 and CD28 proteins simulates the engagement of a T cell with an antigen presenting cell, and results in T cell activation.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

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