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SAPK/JNK Kinase Assay Kit (Nonradioactive)
Cellular Assay Kits

SAPK/JNK Kinase Assay Kit (Nonradioactive) #9810

This product is discontinued

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SAPK/JNK activity of UV, arsenite and anisomycin-treated SK-N-MC cell extracts was analyzed by IP/Kinase assay. Phosphorylation of c-Jun at Ser63 was visualized by immunoblotting with Phospho-c-Jun (Ser63) Antibody

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SAPK-induced phosphorylation of c-Jun was measured by quantitative immunoblotting with Phospho-c-Jun (Ser63) Antibody (A) and compared to direct measurement of phosphate incorporation using [gama-32P]-ATP (B). (Note exposure time difference)

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Product Includes Quantity (with Count)
Kinase Buffer (10X) 9802 1 x 15 ml
Cell Lysis Buffer (10X) 9803 1 x 15 ml
ATP (10 mM) 9804 1 x 50 µl
Anti-rabbit IgG, HRP-linked Antibody 7074 1 x 100 µl
Anti-biotin, HRP-linked Antibody 7075 1 x 100 µl
Biotinylated Protein Ladder Detection Pack 7727 1 x 100 µl
20X LumiGLO® Reagent and 20X Peroxide 7003 2 x 5 ml each
P-c-Jun (S63) Ab (unique to kit) 1 x 100 µl

Nonradioactive SAPK/JNK Assay Kit provides all the reagents necessary to measure SAPK/JNK activity in the cell. A c-Jun fusion protein linked to agarose beads is used to pull down SAPK enzyme from cell extracts. Upon addition of kinase buffer and ATP, SAPK phosphorylates the c-Jun substrate. Phospho-c- Jun (Ser63) Antibody can then be used to measure SAPK activity by immunoblotting.

Species Reactivity:

Human, Mouse, Rat

The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germinal center kinase (GCK) family member (4). There are three SAPK/JNK genes each of which undergoes alternative splicing, resulting in numerous isoforms (3). SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors (3,5).

  1. Davis, R.J. (1999) Biochem Soc Symp 64, 1-12.
  2. Ichijo, H. (1999) Oncogene 18, 6087-93.
  3. Kyriakis, J.M. and Avruch, J. (2001) Physiol Rev 81, 807-69.
  4. Kyriakis, J.M. (1999) J Biol Chem 274, 5259-62.
  5. Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62.
  6. Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem. Sci. 23, 481-485.
Entrez-Gene Id
Swiss-Prot Acc.
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.

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