|23833S||1 Kit (100 tests)||
|Product Includes||Quantity (with Count)|
|Senescence 1X Cell Lysis Buffer||1 x 25 ml|
|Senescence 2X Reaction Buffer||1 x 6.5 ml|
|Senescence Stop Solution||1 x 21 ml|
|SA-β-Gal Substrate||1 x 4 mg|
Additional Reagents (Not Supplied)
|1X PBS Wash||100 µL||400 µL||1000 µL||1500 µL|
|1X Cell Lysis Buffer||100 µL||400 µL||1000 µL||1500 µL|
NOTE: Cell treatments to induce senescence should be completed before initiating assay with this kit. Ensure that an untreated control is included, to provide a baseline measurement of fluorescence for comparison.
2X Assay Buffer Preparation: Immediately before use, add BME to 2X Senescence Reaction Buffer at a final concentration of 10 mM. Then dilute the 20X SA-β-Gal Substrate to 1X with 2X Senescence Reaction Buffer containing 10mM BME. Make only enough solution for the number of wells analyzed (50 µL per well). Use the 2X Assay Buffer immediately and properly discard any unused solution.
posted August 2020
Protocol Id: 2285
β-galactosidase (also known as β-gal) is an essential hydrolase enzyme that catalyzes the hydrolysis of galactose-containing carbohydrates into monosaccharides. Substrates of β-galactosides include lactose, various glycoproteins, ganglioside GM1, and lactosylceramides. β-galactosidase is used widely in molecular biology; for example, isolation of recombinant bacteria during molecular cloning utilizes α-complementation of the bacterial β-galactosidase gene (lacZ) in the presence of a β-gal substrate to identify recombinant clones (1). In cell biology, Senescence-Associated beta-galactosidase (SA-β-gal), defined as β-gal activity at pH 6.0, is a widely used marker of replicative senescence. While initially thought to derive from a unique isoform of β-galactosidase expressed specifically in senescent cells (2), SA-β-gal activity was subsequently shown to result from overexpression and accumulation of β-galactosidase in endogenous lysosomes, and is not specifically required for replicative senescence (3).
UniProt ID: P00722
Entrez-Gene Id: 945006
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.