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9860
Senescence β-Galactosidase Staining Kit

Senescence β-Galactosidase Staining Kit #9860

IC-ABC

β-Galactosidase staining at pH 6 on normal WI38 cells at population doubling 29 (left) and senescent WI38 cells at population doubling 36 (right).

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Β-Galactosidase staining at pH 6.0 on MCF-7 cells untreated (left) and senescent MCF-7 cells treated with etoposide #2200 (12.5 μM, 24 hr) and allowed to recover for 4 days (right).

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Product Includes Quantity (with Count)
10X Fixative Solution 1 x 15 ml
10X Staining Solution 1 x 15 ml
100X Solution A 1 x 1.5 ml
100X Solution B 1 x 1.5 ml
X-Gal 1 x 150 mg

The Senescence β-Galactosidase Staining Kit is designed to conveniently provide reagents needed to detect β-galactosidase activity at pH 6, a known characteristic of senescent cells not found in presenescent, quiescent or immortal cells (3). Papers have published using this kit in both cells and frozen tissue. The kit includes all reagents necessary for this assay.

Limited capacity to replicate is a defining characteristic of most normal cells and culminates in senescence, an arrested state in which the cell remains viable (1). Senescent cells are not stimulated to divide by serum or passage in culture, and senescence invokes a specific cell cycle profile that differs from most damage-induced arrest processes or contact inhibition (2). An enlarged cell size, expression of pH-dependent beta-galactosidase activity (3), and an altered pattern of gene expression (4,5) further characterize senescent cells.

  1. Goldstein, S. (1990) Science 249, 1129-1133.
  2. Sherwood, S. W. et al. (1988) Proc. Natl. Acad. Sci. , USA 85, 9086-9090.
  3. Dimri, G. et al. (1995) Proc. Natl. Acad. Sci., USA 92, 9363-9367.
  4. Cristofalo, V. J. et al. (1998) Crit. Rev. Eukaryot Gene Expr. 8, 43-80.
  5. Linskens, M. H. et al. (1995) Nucleic Acid Res. 23, 3244-3251.
Entrez-Gene Id
945006
Swiss-Prot Acc.
P00722

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For Research Use Only. Not For Use In Diagnostic Procedures.

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To Purchase # 9860S

Product Number Size Price
9860S 1 Kit (125 35mm wells) $215.00.0
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