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Dec 31st Santa Cruz will discontinue a
large number of polyclonal products as
a result of the USDA settlement that was
made public May 19th.

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To Purchase # 9860S

9860S 1 Kit (125 35mm wells) $209.00.0
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Product Includes Quantity (with Count)
10X Fixative Solution 1 x 15 ml
10X Staining Solution 1 x 15 ml
100X Solution A 1 x 1.5 ml
100X Solution B 1 x 1.5 ml
X-Gal 1 x 150 mg

Product Description

The Senescence β-Galactosidase Staining Kit is designed to conveniently provide reagents needed to detect β-galactosidase activity at pH 6, a known characteristic of senescent cells not found in presenescent, quiescent or immortal cells (3). Papers have published using this kit in both cells and frozen tissue. The kit includes all reagents necessary for this assay.


Limited capacity to replicate is a defining characteristic of most normal cells and culminates in senescence, an arrested state in which the cell remains viable (1). Senescent cells are not stimulated to divide by serum or passage in culture, and senescence invokes a specific cell cycle profile that differs from most damage-induced arrest processes or contact inhibition (2). An enlarged cell size, expression of pH-dependent beta-galactosidase activity (3), and an altered pattern of gene expression (4,5) further characterize senescent cells.


1.  Goldstein, S. (1990) Science 249, 1129-1133.

2.  Sherwood, S. W. et al. (1988) Proc. Natl. Acad. Sci. , USA 85, 9086-9090.

3.  Dimri, G. et al. (1995) Proc. Natl. Acad. Sci., USA 92, 9363-9367.

4.  Cristofalo, V. J. et al. (1998) Crit. Rev. Eukaryot Gene Expr. 8, 43-80.

5.  Linskens, M. H. et al. (1995) Nucleic Acid Res. 23, 3244-3251.


Entrez-Gene Id 945006
Swiss-Prot Acc. P00722

Product Citations

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Rader, J. et al. (2013) Clin Cancer Res 19, 6173-82.

Guo, J.Y. et al. (2013) Genes Dev 27, 1447-61.

Evangelou, K. et al. (2013) Cell Death Differ 20, 1485-97.

Khongkow, P. et al. (2014) Oncogene 33, 4144-55.

Flach, J. et al. (2014) Nature 512, 198-202.

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Li, Z. et al. (2013) Oncotarget 4, 2532-49.

Gagou, M.E. et al. (2014) Oncotarget 5, 11381-98.

Zhou, Z.W. et al. (2013) PLoS Genet 9, e1003702.

Sanchez, N. et al. (2013) PLoS One 8, e65671.

Li, L.H. et al. (2014) PLoS One 9, e104203.

Arason, A.J. et al. (2014) PLoS One 9, e88683.

Krencik, R. et al. (2015) Sci Transl Med 7, 286ra66.

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Kannan, A. et al. (2016) Oncotarget 7, 6576-92.

Havel, J.J. et al. (2015) Oncogene 34, 1487-98.

Defamie, V. et al. (2015) Oncogene 34, 4098-108.

Khongkow, P. et al. (2016) Oncogene 35, 990-1002.

Castillo-Lluva, S. et al. (2015) Oncogene 34, 4777-90.

Wan, Y. et al. (2015) J Cell Biol 209, 235-46.

Yuan, Y. et al. (2016) J Biol Chem 291, 1307-19.

Gabitova, L. et al. (2015) Cell Rep 12, 1927-38.

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Chang, J. et al. (2016) Nat Med 22, 78-83.

Baker, D.J. et al. (2016) Nature 530, 184-9.

Takai, H. et al. (2016) Genes Dev 30, 812-26.

Borghesan, M. et al. (2016) Cancer Res 76, 594-606.

De Amicis, F. et al. (2016) Oncotarget , 57955-57969.

Oh, A.Y. et al. (2016) Cancer Res 76, 4791-804.

Lawson, C.D. et al. (2016) Cancer Res 76, 3826-37.

Liceras-Boillos, P. et al. (2016) Oncogene , .

Sriraman, A. et al. (2016) Oncotarget 7, 31623-38.

Lee, H. et al. (2016) Oncotarget 7, 19134-46.

Lowe, D. et al. (2016) Oncotarget 7, 8524-31.

Borghesan, M. et al. (2016) Cancer Res 76, 594-606.

Yuan, Y. et al. (2016) J Biol Chem 291, 1307-19.

Kannan, A. et al. (2016) Oncotarget 7, 6576-92.

Shao, Z. et al. (2016) Nat Commun 7, 10869.

Xiong, Z.M. et al. (2016) Aging Cell 15, 279-90.

Soriano-Arroquia, A. et al. (2016) Aging Cell 15, 361-9.

Llanos, S. et al. (2016) Nat Commun 7, 10438.

Walter, D. et al. (2016) Nat Commun 7, 10530.

Felsenstein, K.M. et al. (2016) ACS Chem Biol 11, 139-48.

Frame, F.M. et al. (2016) Cancer Med 5, 61-73.

Zhu, N. et al. (2016) Stem Cells Int 2016, 5687589.

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Efeyan, A. et al. (2007) Cancer Res 67, 7350-7.

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Baker, D. J. et al. (2004) Nat. Genetics 36, 744-749.

Boucher, M.J. et al. (2004) Am J Physiol Gastrointest Liver Physiol 286, G736-46.

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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

9860
Senescence β-Galactosidase Staining Kit