|Product Includes||Quantity (with Count)|
|10X Fixative Solution||1 x 15 ml|
|10X Staining Solution||1 x 15 ml|
|100X Solution A||1 x 1.5 ml|
|100X Solution B||1 x 1.5 ml|
|X-Gal||1 x 150 mg|
Reagents provided are sufficient to stain 125 x 35 mm wells.
Additional Reagents (Not Supplied)
NOTE: All solutions should be prepared just prior to use.
Volumes are for one 35 mm well of a 6-well plate. Volumes in the procedure should be approximately half that of the tissue culture media. (e.g. 1 ml for 35 mm well/plates containing 2 ml of media, 2.5 ml for 60 mm plates containing 5 ml of media, and 5 ml for 100 mm plates containing 10 ml of media).
IMPORTANT: Always use polypropylene plastic or glass to make and store X-gal. Do not use polystyrene.Dissolve 20 mg of X-gal in 1 ml DMF to prepare a 20 mg/ml stock solution. Excess X-gal solution can be stored in -20°C in a light resistant container for up to one month.
Note: DMSO can be used in place of DMF.
IMPORTANT: Due to variations in water pH, please be sure that the β-Galactosidase Staining Solution has a final pH of 6.0 (A pH 5.9-6.1 is acceptable). pH differences can affect staining: A low pH can result in false positives and high pH can result in false negatives. If necessary, use HCl or NaOH to lower or raise pH, respectively.
Important: Seal plate with parafilm to prevent evaporation. Evaporation can cause crystals to form.
Note: The presence of CO2 can cause changes to the pH which may affect staining results.
posted August 2020
Protocol Id: 2164
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