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7400
Serine/Threonine Kinase Substrate Screening Kit
Cellular Assay Kits
Assay Kit

Serine/Threonine Kinase Substrate Screening Kit #7400

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Serine/Threonine Kinase Substrate Screening Kit: Image 1

Representative Serine/Threonine Kinase Substrate Screening Kit Plate 1 using Akt1 Kinase. Akt1 was used to phosphorylate biotinylated substrate peptides. Vertical-axis is DELFIA® count. Rows are noted by letters A-H. Columns are noted by numbers 1-12. Values in columns 1, 4, 7, and 10 are DELFIA® counts for positive control biotinylated phosphopeptides (shown in yellow). Negative control values (nonphosphopeptides) for the array are found in columns 2, 5, 8, and 11 and shown in blue. Experimental values are found in columns 3, 6, 9, and 12 and shown in red. Positive hits on the array are noted along with partial peptide sequence that served as substrate.

Serine/Threonine Kinase Substrate Screening Kit: Image 2

Kinase of interest is incubated with biotinylated substrate peptides. Phosphorylated products are captured on streptavidin-coated plates. Cell Signaling Technology antibodies serve as primary detection reagents; DELFIA® Europium (Eu) anti-rabbit or anti-mouse chelates are used as secondary antibodies. Positive hits from Serine/Threonine Kinase Substrate Screening Kit (#7400) can be used in any subsequent peptide based kinase assay.

To Purchase # 7400

Product Description

The Serine/Threonine Kinase Substrate Screening Kit provides 87 biotinylated nonphospho-peptides as kinase substrates. Also included are biotinylated phospho and nonphospho-peptides of identical sequence as positive and negative controls respectively. Potential substrate phosphorylation is detected by the corresponding phospho-specific antibodies.

The paired phospho-specific antibody and peptide substrate can be used for high throughput peptide based kinase screens (e.g. FP, TRF, AlphaScreen).

Specificity / Sensitivity

10 to 1000+ fold signal-to-noise ratio of phospho- versus nonphospho-peptides measured by DELFIA® assay.

Background

In order to expedite the isolation of substrates for novel kinases, Cell Signaling Technology has developed a kinase/substrate development kit. Numerous serine/threonine kinases have become pharmaceutically important, including GSK, Akt and p38. With the cloning of hundreds of uncharacterized serine/threonine kinases, drug discovery researchers are seeking to quickly find substrates for high throughput screening programs.

With a total of 87 different peptide/phospho-specific antibody pairs on the array, a very diverse group of substrates is represented. Signal-to-noise ratios have been optimized and range from 10 to over 1000. The phospho-specific antibody and peptide substrate pair can be used for subsequent high throughput peptide based kinase screens (e.g., FP, TRF, AlphaScreen).

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
DELFIA is a registered trademark of PerkinElmer, Inc.
Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at [email protected] For information regarding commercial licensing terms please contact CST Pharma Services Department at [email protected]