Tyrosine Kinase Substrate Screening Kit (#7450) Datasheet Without Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Information

Storage

Antibody is supplied in 50 mM Tris-HCl (pH 7.4), 150 mM NaCl (TBS), 0.05% Tween-20, 1.0 µg/ml BSA. Store at -20°C. Do not aliquot the antibody.
Peptides are supplied at 3 µM in either a 0.001% DMSO solution or a DMSO and carbonate buffer solution. Store peptides at -20°C.

Specificity / Sensitivity

Phospho-Tyrosine mAb (P-Tyr-100) #9411 binds phospho-Tyr in a manner largely independent of the surrounding amino acid sequence, and does not cross-react with peptides containing phospho-Ser or phospho-Thr.
DELFIA^® assay yields 10 to over 100 fold signal-to-noise ratio of phospho- versus nonphospho-peptides .

Source / Purification

Detection antibody source: Phospho-Tyrosine mAb (P-Tyr-100) #9411 is corresponding to mice immunized with phospho-tyrosine-containing peptides .

Product Description

The Tyrosine Kinase Substrate Screenining Kit provides 95 different nonphospho-peptides as kinase substrates. Also included are biotinylated phospho- and nonphospho-peptides of identical sequence to serve as positive and negative controls, respectively. Potential substrate phosphorylation is detected by phospho-tyrosine specific antibody #9411, provided in the kit.

Background

Numerous tyrosine kinases have become pharmaceutically important. With the cloning of hundreds of uncharacterized tyrosine kinases, drug discovery researchers are seeking to quickly find substrates for high throughput screening programs. To expedite the isolation of substrates for novel kinases, Cell Signaling Technology has developed kinase/substrate screening kits.
With a total of 95 different peptides on the array, a very diverse group of substrates is represented. Signal-to-noise ratios have been optimized and range from 10 to over 100. The phosphotyrosine-specific antibody and peptide substrate can be used for subsequent high throughput peptide based kinase screens (e.g., FP, TRF, AlphaScreen).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

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