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7450
Tyrosine Kinase Substrate Screening Kit
Cellular Assay Kits
Assay Kit

Tyrosine Kinase Substrate Screening Kit #7450

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Tyrosine Kinase Substrate Screening Kit: Image 1
Treatment of Tyrosine Kinase Substrate Screening Kit with Met kinase: Positive control is shown in yellow, and negative control is shown in blue. Several positive hits were identified using this approach (in red).
Tyrosine Kinase Substrate Screening Kit: Image 2
Treatment of Tyrosine Kinase Substrate Screening Kit with Src kinase: Positive control is shown in yellow, and negative control is shown in blue. Several positive hits were identified using this approach (in red).
Tyrosine Kinase Substrate Screening Kit: Image 3
Kinase of interest is incubated with biotinylated substrate peptides. Phosphorylated products are captured on streptavidin-coated plates. Cell Signaling Technology, Inc. antibody #9411 P-Tyr-100 serves as the primary detection reagent; DELFIA® Europium (Eu) anti-mouse chelates are used as secondary antibodies. Positive hits from Tyrosine Kinase Substrate Screening Kit #7450 can be used in any subsequent peptide based kinase assay.
Inquiry Info.# 7450

Product Description

The Tyrosine Kinase Substrate Screenining Kit provides 95 different nonphospho-peptides as kinase substrates. Also included are biotinylated phospho- and nonphospho-peptides of identical sequence to serve as positive and negative controls, respectively. Potential substrate phosphorylation is detected by phospho-tyrosine specific antibody #9411, provided in the kit.

Specificity / Sensitivity

Phospho-Tyrosine mAb (P-Tyr-100) #9411 binds phospho-Tyr in a manner largely independent of the surrounding amino acid sequence, and does not cross-react with peptides containing phospho-Ser or phospho-Thr. DELFIA® assay yields 10 to over 100 fold signal-to-noise ratio of phospho- versus nonphospho-peptides .

Background

Numerous tyrosine kinases have become pharmaceutically important. With the cloning of hundreds of uncharacterized tyrosine kinases, drug discovery researchers are seeking to quickly find substrates for high throughput screening programs. To expedite the isolation of substrates for novel kinases, Cell Signaling Technology has developed kinase/substrate screening kits. With a total of 95 different peptides on the array, a very diverse group of substrates is represented. Signal-to-noise ratios have been optimized and range from 10 to over 100. The phosphotyrosine-specific antibody and peptide substrate can be used for subsequent high throughput peptide based kinase screens (e.g., FP, TRF, AlphaScreen).

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
DELFIA is a registered trademark of PerkinElmer, Inc.
Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at [email protected]al.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at [email protected]