Use as directed in the CUT&RUN Assay Kit #86652 protocol. In detail, we suggest adding 3 μl 10% SDS Solution and an appropriate amount of Proteinase K (20 mg/ml) #10012 to each 300 μl CUT&RUN reaction to reverse formaldehyde crosslinks. This product can also be used in other assays at required amounts. Please make sure the 10% SDS Solution is completely in solution before use. If the product is not completely in solution, warm it up to ~50°C.
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