For the CUT&RUN assay, we recommend adding 37.5 μl CUT&RUN 4X Stop Buffer, 3.75 μl Digitonin Solution #16359, and 0.75 μl RNAse A (10 mg/ml) #7013 to 108 μl nuclease-free water (150 μl per reaction) right before use. Then incubate with the sample at 37°C for 10 min. An appropriate amount of Sample Normalization Spike-In DNA can be added to CUT&RUN 4X Stop Buffer, if desired.
Store CUT&RUN 4X Stop Buffer at -20°C. This product is stable for at least 12 months.
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