For the CUT&RUN assay, we recommend adding 2 μl Proteinase K (20 mg/ml) #10012 and 0.5 μl RNAse A (10 mg/ml) #7013 to 197.5 μl CUT&RUN DNA Extraction Buffer (200 μl per input sample) right before use. Then incubate with a 100 μl cell suspension at 55°C for 1 hr with shaking to extract genomic DNA.
Store CUT&RUN DNA Extraction Buffer at 4°C. This product is stable for at least 12 months.
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