10.1
#P15514
374
Background
Amphiregulin (AREG) is essential for mammary ductal development and is expressed in many parts of the body, such as the ovary, placenta, pancreas, breast, lung, and spleen (1,2). Studies on preneoplastic human breast tissue and breast cancer cell lines suggest a significant role for amphiregulin in driving human breast cancer progression (1). Amphiregulin interacts with the epidermal growth factor (EGF) receptor to promote the growth of normal epithelial cells (3). It drives the proliferation of fibroblasts and production of cytokines interleukin 8 (IL-8) and vascular endothelial growth factor (VEGF) which suggests a proinflammatory role as observed in rheumatoid arthritis patients (4).
Endotoxin
Endotoxin levels are less than or equal to 1 EU / 1 μg hAmphiregulin.
Purity
A greater than or equal to 95% purity was determined by SDS-PAGE.
Source / Purification
Recombinant human Amphiregulin was expressed in E. coli and is supplied in a lyophilized form.
Bioactivity
The bioactivity of recombinant hAmphiregulin was determined in a 3T3 cell proliferation assay. The ED50 of each lot is less than or equal to 20 ng/mL.
Background
Amphiregulin (AREG) is essential for mammary ductal development and is expressed in many parts of the body, such as the ovary, placenta, pancreas, breast, lung, and spleen (1,2). Studies on preneoplastic human breast tissue and breast cancer cell lines suggest a significant role for amphiregulin in driving human breast cancer progression (1). Amphiregulin interacts with the epidermal growth factor (EGF) receptor to promote the growth of normal epithelial cells (3). It drives the proliferation of fibroblasts and production of cytokines interleukin 8 (IL-8) and vascular endothelial growth factor (VEGF) which suggests a proinflammatory role as observed in rheumatoid arthritis patients (4).
Background References
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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