11.2
#P29279
1490
Background
Connective tissue growth factor (CTGF, CCN2) belongs to the CCN (CYR61, CTGF, NOV) family of secreted extracellular matrix (ECM) proteins (1). Members of this family contain four conserved cysteine-rich domains, and interact in the ECM with a diverse array of cell surface receptors, including integrins and heparin-sulfate proteoglycans (2). These interactions regulate a multitude of cellular and tissue functions, including adhesion, proliferation, migration, differentiation, senescence, angiogenesis, inflammation, and wound repair (1,3-5). The CTGF gene is a transcriptional target of both YAP/TAZ and TGFβ-SMAD signaling pathways (6,7), and aberrant regulation of CTGF expression is strongly associated with pathological conditions, notably cancer and fibrosis (8,9).
- Chen, C.C. and Lau, L.F. (2009) Int J Biochem Cell Biol 41, 771-83.
- Gao, R. and Brigstock, D.R. (2004) J Biol Chem 279, 8848-55.
- Hall-Glenn, F. and Lyons, K.M. (2011) Cell Mol Life Sci 68, 3209-17.
- Hashimoto, G. et al. (2002) J Biol Chem 277, 36288-95.
- Ivkovic, S. et al. (2003) Development 130, 2779-91.
- Di Benedetto, A. et al. (2016) Oncotarget 7, 43188-43198.
- Liu, H. et al. (2015) Cell Biochem Biophys 73, 775-81.
- Brigstock, D.R. (2010) J Cell Commun Signal 4, 1-4.
- Jun, J.I. and Lau, L.F. (2011) Nat Rev Drug Discov 10, 945-63.
Endotoxin
Endotoxin levels are less than or equal to 1 EU / 1 μg hCTGF.
Purity
A greater than or equal to 90% purity was determined by SDS-PAGE.
Source / Purification
Recombinant human CTGF was expressed in E. coli and is supplied in a lyophilized form.
Bioactivity
The bioactivity of recombinant hCTGF was determined in a HUVEC cell proliferation assay. The ED50 of each lot is less than or equal to 2,000 ng/mL.
Background
Connective tissue growth factor (CTGF, CCN2) belongs to the CCN (CYR61, CTGF, NOV) family of secreted extracellular matrix (ECM) proteins (1). Members of this family contain four conserved cysteine-rich domains, and interact in the ECM with a diverse array of cell surface receptors, including integrins and heparin-sulfate proteoglycans (2). These interactions regulate a multitude of cellular and tissue functions, including adhesion, proliferation, migration, differentiation, senescence, angiogenesis, inflammation, and wound repair (1,3-5). The CTGF gene is a transcriptional target of both YAP/TAZ and TGFβ-SMAD signaling pathways (6,7), and aberrant regulation of CTGF expression is strongly associated with pathological conditions, notably cancer and fibrosis (8,9).
Background References
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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