25.8
#P10600
7043
Background
TGF-β3 can induce cell proliferation and angiogenesis, and can promote some immune events while inhibiting others (1-3). TGF-β3 is produced by chondrocytes, glial cells, astrocytes, and some cancer cells (3,4). TGF-β3 binds to TβRII homodimer, which then complexes with TβRI homodimer (1,5). The oligomeric receptor complex phosphorylates subsets of SMAD proteins that then act to induce or suppress target genes (1,5). Based on knockout studies in mice, TGF-β3 is critical for lung and palate development (6).
- Siegel, P.M. and Massagué, J. (2003) Nat Rev Cancer 3, 807-21.
- Bierie, B. and Moses, H.L. (2006) Nat Rev Cancer 6, 506-20.
- Damstrup, L. et al. (1993) Br J Cancer 67, 1015-21.
- Constam, D.B. et al. (1992) J Immunol 148, 1404-10.
- Moustakas, A. and Heldin, C.H. (2009) Development 136, 3699-714.
- Dünker, N. and Krieglstein, K. (2000) Eur J Biochem 267, 6982-8.
Endotoxin
Endotoxin levels are less than or equal to 1 EU / 1 μg h/mTGF-β.
Purity
A greater than or equal to 95% purity was determined by SDS-PAGE.
Source / Purification
Recombinant human/mouse TGF-β3 was expressed in E. coli and is supplied in a lyophilized form.
Bioactivity
The bioactivity of recombinant h/mTGF-β3 was determined in an IL-4 induced HT-2 cell proliferation assay. The ED50 of each lot is less than or equal to 0.038 ng/mL.
Background
TGF-β3 can induce cell proliferation and angiogenesis, and can promote some immune events while inhibiting others (1-3). TGF-β3 is produced by chondrocytes, glial cells, astrocytes, and some cancer cells (3,4). TGF-β3 binds to TβRII homodimer, which then complexes with TβRI homodimer (1,5). The oligomeric receptor complex phosphorylates subsets of SMAD proteins that then act to induce or suppress target genes (1,5). Based on knockout studies in mice, TGF-β3 is critical for lung and palate development (6).
Background References
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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