Mouse IL-12 Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.
|Purity||A greater than or equal to 95% purity was determined by SDS-PAGE.|
|Endotoxin||Endotoxin levels are less than or equal to 1 EU / 1 μg mIL-12.|
|Activity||The bioactivity of recombinant mIL-12 was determined in a secreted embryonic alkaline phosphatase (SEAP) induction assay using HEK-BlueTM IL-12 cells. The ED50 of each lot is less than or equal to 15 ng/ml. HEK-BlueTM IL-12 cells were purchased from InvivoGen Inc.|
Recombinant mouse IL-12 was expressed in genetically modified HEK 293 cells and is supplied in a lyophilized form.
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