#P18065
3485
Background
IGFBP2 is a multifunctional protein that plays a key role in the regulation of IGFI/II activity, cell proliferation, and cell adhesion (1,2). One of six high-affinity IGF binding proteins, IGFBP2 is the second most abundant IGFBP species in circulation (1). IGFBP2 can antagonize IGF signaling, or directly stimulate cell proliferation depending on context and cell type (1,2). Many of these effects are IGF independent (2). Elevated serum levels of IGFBP2 have been reported in a variety of cancers (1,2). IGFBP2 has been implicated in angiogenesis in a murine melanoma model (3).
Endotoxin
Less than 0.01 ng endotoxin/1 μg hHis6IGFBP2.
Purity
>95% as determined by SDS-PAGE of 6 μg reduced (+) and nonreduced (-) recombinant hHisIGFBP2. All lots are greater than 95% pure.
Source / Purification
Recombinant Human His6IGFBP2 (hHis6IGFBP2) Glu40-Gln328 (Accession #NP_18065) was expressed in human 293 cells at Cell Signaling Technology.
Bioactivity
The bioactivity of hHis6IGFBP2 was determined by inhibition of IGF-I induced AKT phosphorylation in human dermal fibroblasts. The ED50 of each lot is between 0.1 and 0.5 µg/ml.
Background
IGFBP2 is a multifunctional protein that plays a key role in the regulation of IGFI/II activity, cell proliferation, and cell adhesion (1,2). One of six high-affinity IGF binding proteins, IGFBP2 is the second most abundant IGFBP species in circulation (1). IGFBP2 can antagonize IGF signaling, or directly stimulate cell proliferation depending on context and cell type (1,2). Many of these effects are IGF independent (2). Elevated serum levels of IGFBP2 have been reported in a variety of cancers (1,2). IGFBP2 has been implicated in angiogenesis in a murine melanoma model (3).
Background References
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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