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9952
Basic Fibroblast Growth Factor (bFGF)
Cytokines

Basic Fibroblast Growth Factor (bFGF) #9952

This product is discontinued

Western Blotting Image 1

Western blot analysis of extracts from NIH/3T3 cells, untreated or bFGF-stimulated (100 ng/ml for 10 minutes), using Phospho-FRS-2 alpha (Tyr436) Antibody.

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Human Recombinant Protein

The human bFGF coding cDNA was obtained from human bone marrow cell mRNA, subcloned into a prokaryotic expression vector and expressed in E. coli. The recombinant human bFGF protein was purified and stored in PBS buffer containing 0.1%BSA.

Purity:

>97%

Bioactivity:

The EC50 for stimulation of Balb/c 3T3 cell proliferation is 0.1-0.2 ng/ml.

CST recommends using 50-100 ng/ml for stimulation of bFGF signaling.

Storage:

Basic fibroblast growth factor (bFGF) is supplied as a solution. It should be stored at –80°C. Aliquot the reagent upon receipt and avoid repeated freeze-thaw cycles.

Fibroblast growth factors are a family of broad-spectrum growth factors influencing a plethora of cellular activities. The interaction of at least 23 ligands, 4 receptors and multiple coreceptors provides a dramatic complexity to a signaling system capable of effecting a multitude of responses (1,2). Basic fibroblast growth factor (bFGF or FGF2), initially identified as a mitogen with prominent angiogenic properties, is now recognized as a multifunctional growth factor (3). It is clear that bFGF produces its biological effects in target cells by signaling through cell-surface FGF receptors. bFGF binds to all four FGF receptors. Ligand binding induces receptor dimerization and autophosphorylation, allowing binding and activation of cytoplasmic downstream target proteins, including FRS-2, PLC and Crk (4,5). The FGF signaling pathway appears to play a significant role not only in normal cell growth regulation but also in tumor development and progression (6).

Acidic FGF (aFGF or FGF1) is another extensively investigated protein of the FGF family. aFGF shares 55% DNA sequence homology with bFGF. These two growth factors are ubiquitously expressed and exhibit a wide spectrum of similiar biological activities with quantitative differences likely due to variation in receptor affinity or binding (7).

The human bFGF coding cDNA was obtained from human bone marrow cell mRNA, subcloned into a prokaryotic expression vector and expressed in E. coli. The recombinant human bFGF protein was purified and stored in PBS buffer containing 0.1%BSA.

  1. Powers, C.J. et al. (2000) Endocrine-Related Cancer 7, 165-197.
  2. Bansal, R. (2002) Dev. Neurosci. 24, 35-46.
  3. Morrison, R.S. et al. (1994) J. Neurooncol. 18, 207-216.
  4. Kouhara, H. et al. (1997) Cell 89, 693-702.
  5. Mohammadi, M. et al. (1991) Mol. Cell. Biol. 11, 5068-5078.
  6. Bikfalvi, A. (1995) Eur. J. Cancer 31A, 1101-1104.
  7. Ledoux, D. et al. (1992) Prog. Growth Factor Res. 4, 107-120.
Entrez-Gene Id
2247
Swiss-Prot Acc.
P09038
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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