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9952
Basic Fibroblast Growth Factor (bFGF)
Cytokines
Growth Factors and Cytokines

Basic Fibroblast Growth Factor (bFGF) #9952

Citations (0)
Basic Fibroblast Growth Factor (bFGF): Image 1

Western blot analysis of extracts from NIH/3T3 cells, untreated or bFGF-stimulated (100 ng/ml for 10 minutes), using Phospho-FRS-2 alpha (Tyr436) Antibody.

Product Usage Information

CST recommends using 50-100 ng/ml for stimulation of bFGF signaling.

Storage

Basic fibroblast growth factor (bFGF) is supplied as a solution. It should be stored at –80°C. Aliquot the reagent upon receipt and avoid repeated freeze-thaw cycles.

Product Description

The human bFGF coding cDNA was obtained from human bone marrow cell mRNA, subcloned into a prokaryotic expression vector and expressed in E. coli. The recombinant human bFGF protein was purified and stored in PBS buffer containing 0.1%BSA.

Purity

>97%

Source / Purification

Human Recombinant Protein

Background

Fibroblast growth factors are a family of broad-spectrum growth factors influencing a plethora of cellular activities. The interaction of at least 23 ligands, 4 receptors and multiple coreceptors provides a dramatic complexity to a signaling system capable of effecting a multitude of responses (1,2). Basic fibroblast growth factor (bFGF or FGF2), initially identified as a mitogen with prominent angiogenic properties, is now recognized as a multifunctional growth factor (3). It is clear that bFGF produces its biological effects in target cells by signaling through cell-surface FGF receptors. bFGF binds to all four FGF receptors. Ligand binding induces receptor dimerization and autophosphorylation, allowing binding and activation of cytoplasmic downstream target proteins, including FRS-2, PLC and Crk (4,5). The FGF signaling pathway appears to play a significant role not only in normal cell growth regulation but also in tumor development and progression (6).

Acidic FGF (aFGF or FGF1) is another extensively investigated protein of the FGF family. aFGF shares 55% DNA sequence homology with bFGF. These two growth factors are ubiquitously expressed and exhibit a wide spectrum of similiar biological activities with quantitative differences likely due to variation in receptor affinity or binding (7).

The human bFGF coding cDNA was obtained from human bone marrow cell mRNA, subcloned into a prokaryotic expression vector and expressed in E. coli. The recombinant human bFGF protein was purified and stored in PBS buffer containing 0.1%BSA.

  1. Powers, C.J. et al. (2000) Endocrine-Related Cancer 7, 165-197.
  2. Bansal, R. (2002) Dev. Neurosci. 24, 35-46.
  3. Morrison, R.S. et al. (1994) J. Neurooncol. 18, 207-216.
  4. Kouhara, H. et al. (1997) Cell 89, 693-702.
  5. Mohammadi, M. et al. (1991) Mol. Cell. Biol. 11, 5068-5078.
  6. Bikfalvi, A. (1995) Eur. J. Cancer 31A, 1101-1104.
  7. Ledoux, D. et al. (1992) Prog. Growth Factor Res. 4, 107-120.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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