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CD40 Ligand
Growth Factors and Cytokines

CD40 Ligand #3064

Citations (0)

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# Product Name Applications Reactivity
CD40 Ligand: Image 1
Western blot analysis of extracts from T-47D cells, untreated or treated with CD40L at the indicated concentrations for 15 minutes, using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033 (upper), Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) Rabbit mAb #4370 (middle) and Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb #4668 (lower).

Product Usage Information

The working concentration of CD40L generally ranges from 10-50 ng/ml.


Lyophilized product is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml, which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.

Product Description

Recombinant mature human CD40L (amino acids 113-261) was expressed in E. coli and is supplied in a lyophilized form. A greater than 98% purity was determined by reverse-phase HPLC and SDS-PAGE.
MW (kDa) 16.3
Purity >98%

Source / Purification

E. coli


CD40 ligand (CD40L), also known as CD154, TRAP, and gp39, is the ligand for the TNF receptor family member CD40 (1-6). CD40L is expressed either as a soluble cytokine or as a homotrimeric transmembrane protein. CD40L primarily expressed on the surface of T-cells, but has also been reported in blood platelets, mast cells, basophils, NK cells, and B-cells. It plays an important role in stimulating B-cell cell proliferation and survival and promotes immunoglobulin class switching and secretion of IgE (7). Signals generated by CD40 vary depending on cell type and include activation of MAPK pathways as well as NF-κB (8-11). Mutations within the CD40L gene are associated with X-linked hyper-IgM syndrome characterized by high serum levels of IgM and decreased levels of other isotypes (12). The CD40L/CD40 pathway is an important area of interest in the study of cancer, vascular diseases, and inflammatory disorders (13-15).
  1. Noelle, R.J. et al. (1992) Proc Natl Acad Sci USA 89, 6550-4.
  2. Graf, D. et al. (1992) Eur J Immunol 22, 3191-4.
  3. Armitage, R.J. et al. (1992) Nature 357, 80-2.
  4. Hollenbaugh, D. et al. (1992) EMBO J 11, 4313-21.
  5. Gauchat, J.F. et al. (1993) FEBS Lett 315, 259-66.
  6. Castle, B.E. et al. (1993) J Immunol 151, 1777-88.
  7. Spriggs, M.K. et al. (1992) J Exp Med 176, 1543-50.
  8. Li, Y.Y. et al. (1996) J Immunol 157, 1440-7.
  9. Pearson, L.L. et al. (2001) Int Immunol 13, 273-83.
  10. Berberich, I. et al. (1996) EMBO J 15, 92-101.
  11. Aruffo, A. et al. (1993) Cell 72, 291-300.
  12. Berberich, I. et al. (1994) J Immunol 153, 4357-66.
  13. Korniluk, A. et al. (2014) Tumour Biol 35, 9447-57.
  14. Hassan, G.S. et al. (2012) Immunobiology 217, 521-32.
  15. Zhang, B. et al. (2013) Immunol Lett 153, 58-61.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.
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