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9908
Epidermal Growth Factor (Human EGF)
Cytokines
Growth Factors and Cytokines

Epidermal Growth Factor (Human EGF) #9908

Citations (0)

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# Product Name Application Reactivity
Epidermal Growth Factor (Human EGF): Image 1

Western blot analysis of extracts from A431 cells, untreated or EGF-stimulated (100 ng/ml for 2 minutes), using Phospho-EGF Receptor (Tyr1068) Antibody #2234 (upper) or EGF Receptor Antibody #2232 (lower).

Product Usage Information

CST recommends using 50-100 ng/ml of Human EGF for stimulation of Human EGF signaling.

Storage

Human EGF is supplied as a solution. It should be stored at –80°C. Aliquot the reagent upon receipt and avoid repeat freeze-thaw cycles.

Product Description

The human EGF coding cDNA was obtained from human periodontal tissue mRNA, subcloned into a prokaryotic expression vector and expressed in E. coli. Epidermal Growth Factor (Human EGF) was purified and stored in PBS buffer containing 0.1% BSA.

Purity

>98%

Source / Purification

Human Recombinant Protein

Background

Epidermal growth factor (EGF) is a small polypeptide hormone that has mitogenic properties in vivo and in vitro and effects the growth and/or differentiation of many cell types. EGF elicits biologic responses by binding to its cell surface receptor, which is a transmembrane glycoprotein containing a cytoplasmic protein tyrosine kinase (1,2). The binding of EGF to EGF receptor induces dimerization of the receptor, autophosphorylation, and activation of downstream signaling components (3). The integrated biological responses to EGF signaling are pleiotropic, including mitogenesis or apoptosis, enhanced cell motility, protein secretion, and differentiation or dedifferentiation. In addition to being implicated in organ morphogenesis, maintenance and repair, research studies have correlated upregulated EGF receptor signaling with progression to invasion and metastasis in a wide variety of tumors (4-6). Thus, investigator have identified EGF receptor and its downstream signaling molecules as targets for therapeutic interventions in wound repair and cancer (4-6).

  1. Wells, A. (1999) Int J Biochem Cell Biol 31, 637-43.
  2. Boulougouris, P. and Elder, J. Anticancer Res 21, 2769-75.
  3. Schlessinger, J. (2002) Cell 110, 669-72.
  4. Sarries, C. et al. (2002) Pharmacogenomics 3, 763-80.
  5. Lorimer, I.A. (2002) Curr Cancer Drug Targets 2, 91-102.
  6. Ghaneh, P. et al. (2002) J Hepatobiliary Pancreat Surg 9, 1-11.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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