Interested in promotions? | Click here >>
89628
Human BAFF/TNFSF13B (hBAFF)
Cytokines
Growth Factors and Cytokines

Human BAFF/TNFSF13B (hBAFF) #89628

Reviews ()
Citations (0)
Human BAFF/TNFSF13B (hBAFF): Image 1

RPMI 8226 cells were cultured with 0 to 500 ng/mL of hBAFF in the presence of 0.1 μM dexamethasone. Cell proliferation was assessed after 91 hours by measuring the OD490.

Human BAFF/TNFSF13B (hBAFF): Image 2

The purity of recombinant hBAFF was determined by SDS-PAGE of 1.5 µg reduced (+) and non-reduced (-) recombinant hBAFF and staining overnight with Coomassie Blue.

Storage

Recombinant human BAFF is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long term storage.

Product Description

Purity

> 90%

Source / Purification

Recombinant human BAFF was expressed in E. coli and is supplied in a lyophilized form. A greater than 90% purity was determined by SDS-PAGE. Endotoxin levels are less than or equal to 1 EU / 1 μg hBAFF.

Background

BAFF, a member of the TNF superfamily of proteins, is a homotrimeric transmembrane protein, which is cleaved to produce a soluble cytokine (1). BAFF may also further oligomerize into 60-mer structures (1). BAFF is expressed by neutrophils, macrophages, dendritic cells, activated T cells, and epithelial cells (1,2). BAFF plays a key role in B cell development, survival, and activation (1,3,4). BAFF binds to three distinct receptors, BAFF-R, TACI, and BCMA (1). These receptors are differentially expressed during B cell development and among B cell subsets (1,2,4). While BAFF-R and BCMA bind to the homotrimeric form of BAFF, TACI only binds to membrane bound or higher order BAFF structures (1). The BAFF/ BAFF-R interaction activates both canonical and non-canonical NF-κB pathways, PI3K/Akt, and mTOR (2,4). Activation of the noncanonical NF-κB pathway via BAFF-R is negatively regulated by TRAF3 (5). Elevated levels of BAFF may exacerbate many autoimmune disorders, making it an attractive therapeutic target (2).

  1. Mackay, F. and Schneider, P. (2009) Nat Rev Immunol 9, 491-502.
  2. Moisini, I. and Davidson, A. (2009) Clin Exp Immunol 158, 155-63.
  3. Schiemann, B. et al. (2001) Science 293, 2111-4.
  4. Khan, W.N. (2009) J Immunol 183, 3561-7.
  5. Gardam, S. et al. (2008) Immunity 28, 391-401.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
To Purchase # 89628S
Product # Size Price
89628S
20 µg $ 240