Serial dilutions of Human FGF basic (hFGF basic/FGF2), starting at 500 ng/mL, were added to NIH/3T3 cells. Cell proliferation was assessed after 48 hours by measuring OD490.
The purity of recombinant hFGF basic was determined by SDS-PAGE of 1.5 µg reduced (+) and non-reduced (-) recombinant hFGF basic and staining overnight with Coomassie Blue.
Western blot analysis of extracts from NIH/3T3 cells, untreated or treated with Human Basic Fibroblast Growth Factor for 20 minutes, using Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb #9154 (upper) and MEK1/2 (47E6) Rabbit mAb #9126 (lower).
Working concentration of hFGF basic generally ranges from 0.1-10 ng/ml.
Recombinant human FGF basic is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long term storage.
A greater than 95% purity was determined by SDS-PAGE.
Less than or equal to 1 EU / 1 μg hFGF basic
The bioactivity of recombinant hFGF basic was determined in a NIH/3T3 cell proliferation assay. The ED50 of each lot is between 0.05 - 1.0 ng/ml.
Recombinant human FGF basic was expressed in E. coli and is supplied in a lyophilized form.
FGF basic (FGF2) is produced by epithelial, tumor and other cell types (1). FGF basic is involved in developmental processes and regulates differentiation, proliferation, and migration (1-6). FGF basic is a critical factor for growing embryonic stem cells in culture without inducing differentiation. FGF basic has a high affinity for heparan sulfate (1,2) and FGF-heparan sulfate binding is a step in the activation of FGFR tyrosine kinase. There are four distinct FGF receptors and each has multiple splice variants. FGF basic binds with high affinity to many, but not all, FGFRs. Signaling cascades activated through FGF basic binding to FGFR include the ras-raf-MAPK, PLCγ/PKC, and PI3K/AKT pathways (1).
Explore pathways + proteins related to this product.
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.