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4697
Human BMP-2 Recombinant Protein
Cytokines & Growth Factors
Growth Factors and Cytokines

Human BMP-2 Recombinant Protein #4697

Citations (3)
ATDC-5 cells were cultured with 0 to 1000 ng/mL of recombinant hBMP-2. Alkaline phosphatase activity was measured after 67 hours by measuring the OD405.
The purity of recombinant hBMP-2 was determined by SDS-PAGE of 1 µg reduced (+) and non-reduced (-) recombinant hBMP-2 and staining overnight with Coomassie Blue.
Western blot analysis of extracts from serum-starved HT-1080 cells, untreated and treated for 30 minutes with various concentrations of recombinant hBMP-2, using Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb #9516 (upper) and Smad1 Antibody #9743 (lower).

Product Usage Information

¶The working concentration of recombinant human BMP-2 generally ranges from 20-50 ng/ml.

Storage

Lyophilized product is very stable at -20°C. It is recommended to reconstitute with 10 mM HCl at a concentration of 0.1 mg/ml, which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.

Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.

Product Description

MW (kDa) 26
Purity >95%
Activity The ED50 as determined by the cytolysis of MC3T3-E1 cells is less than 50 ng/ml.

Source / Purification

Recombinant human BMP-2 was expressed in E. coli and is supplied in a lyophilized form.

Background

Bone morphogenetic proteins (BMPs) were first identified as molecules that can induce ectopic bone and cartilage formation (1,2). BMPs are synthesized as precursor proteins that are processed by cleavage to produce mature proteins. BMPs initiate signaling by binding to a receptor complex containing type I and type II serine/threonine receptor kinases that then phosphorylate Smad (mainly Smad1, 5 and 8), resulting in the translocation of Smad to the nucleus. BMP was also reported to activate MAPK pathways in some systems (3,4).

Pathways

Explore pathways related to this product.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
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To Purchase # 4697
Cat. # Size Qty. Price
4697S
10 µg