Working concentration of hCD40L generally ranges from 1-100 ng/ml.
Recombinant human CD40L is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml, which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long term storage.
|Purity||A greater than 95% purity was determined by SDS-PAGE.|
|Endotoxin||Less than or equal to 1 EU / 1 μg hCD40L.|
|Activity||The bioactivity of recombinant hCD40L was determined by measuring the secreted embryonic alkaline phosphatase (SEAP) activity in HEK-blue™ CD40L responsive indicator cells. The ED50 of each lot is between 5-50 ng/ml.|
Recombinant human CD40L was expressed in E. coli and is supplied in a lyophilized form.
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