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32621
Human CD40 Ligand (hCD40L)
Cytokines
Growth Factors and Cytokines

Human CD40 Ligand (hCD40L) #32621

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Human CD40 Ligand (hCD40L): Image 1

HEK-blue™ CD40L responsive indicator cells were cultured with 0 to 2 μg/ml of hCD40L. Secreted embryonic alkaline phosphatase (SEAP) activity was assessed by measuring OD650.

Human CD40 Ligand (hCD40L): Image 2

The purity of recombinant hCD40L was determined by SDS-PAGE of 1.5 µg reduced (+) and non-reduced (-) recombinant hCD40L and staining overnight with Coomassie Blue.

Human CD40 Ligand (hCD40L): Image 3

Western blot analysis of extracts from THP-1 cells, untreated or treated with hCD40L for 15 minutes, using Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb #4668 (upper) and SAPK/JNK Antibody #9252 (lower).

Product Usage Information

Working concentration of hCD40L generally ranges from 1-100 ng/ml.

Storage

Recombinant human CD40L is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml, which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long term storage.

Product Description

Purity

A greater than 95% purity was determined by SDS-PAGE.

Source / Purification

Recombinant human CD40L was expressed in E. coli and is supplied in a lyophilized form. A greater than 95% purity was determined by SDS-PAGE. Endotoxin levels are less than or equal to 1 EU / 1 μg hCD40L.

Background

CD40 Ligand (CD40L), a member of the TNF superfamily of ligands, is expressed as either a membrane-bound or soluble homotrimer (1). Both membrane-bound and soluble forms of CD40L have biological activity (1,2). CD40L is expressed primarily on activated T cells; however, mast cells, basophils, and NK cells may also express CD40L (1). CD40L functions as an important T cell co-stimulatory molecule and enhances T-dependent B cell responses (1,2). CD40L binds CD40, which is a receptor expressed on B cells, dendritic cells, and macrophages (1). Binding of CD40L to CD40 leads to the recruitment of TRAF proteins (TRAFs 2,3,5, and 6) resulting in the activation of the JNK and NF-κB pathways (1,2).

  1. van Kooten, C. and Banchereau, J. (2000) J Leukoc Biol 67, 2-17.
  2. Peters, A.L. et al. (2009) Semin Immunol 21, 293-300.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
To Purchase # 32621S
Product # Size Price
32621S
50 µg $ 240