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8012
Human His6Thymic Stromal Lymphopoietin (hHis6TSLP)

Human His6Thymic Stromal Lymphopoietin (hHis6TSLP) #8012

This product is discontinued

Coomassie Gel Image 1

The purity of recombinant hHis6TSLP was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hHisTSLP and staining overnight with Coomassie Blue.

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Bioactivity Image 2

TARC production from human PBMC treated with increasing concentrations of hHis6TSLP was assessed. After 24 hours, cell supernatants were harvested and assayed for TARC by ELISA and the OD450-OD650 was determined.

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Recombinant human His6TSLP (hHis6TSLP) Tyr29-Gln159 (Accession #NP_149024) was expressed in human 293 cells at Cell Signaling Technology. Recombinant hHis6TSLP contains 2 point mutations to eliminate potential furin cleavage sites (R127A and R130S).

Purity:

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hHis6TSLP. All lots are greater than 98% pure.

Molecular Formula:

Recombinant human N-terminally His6-tagged TSLP has a calculated MW of 17,352. DTT-reduced and non-reduced protein migrate as 31 kDa polypeptides. Lower mobility in SDS-PAGE is due to glycosylation. The expected amino terminus of recombinant hHis6TSLP was verified by amino acid sequencing.

Bioactivity:

The bioactivity of recombinant hHis6TSLP was determined by its ability to induce TARC production by PBMC. The ED50 of each lot is between 50-200 pg/ml.

Endotoxin:

Less than 0.01 ng endotoxin/1 μg hHis6TSLP.

Formulation:

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hHis6TSLP. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Storage:

Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

TSLP is an IL-7-like cytokine that plays a key role in promoting TH2–type immune responses (1). Epithelial cells in the skin, lung, and gut are the predominant producers of TSLP (1). A number of immune cell types are responsive to TSLP including dendritic cells, lymphocytes, mast cells, and basophils, among others (1). Consistent with its role as a promoter of TH2-type immune responses, TSLP is a crucial component of the anti-helminth immune response (1). Moreover, TSLP may promote tumor progression by inducing TH2-type inflammation (2-4). TSLP signals through IL-7Rα/TSLPR heterodimers, activating Jak1/2 and Stat5 pathways (5,6).

  1. Ziegler, S.F. and Artis, D. (2010) Nat Immunol 11, 289-93.
  2. Pedroza-Gonzalez, A. et al. (2011) J Exp Med 208, 479-90.
  3. De Monte, L. et al. (2011) J Exp Med 208, 469-78.
  4. Olkhanud, P.B. et al. (2011) J Immunol 186, 5656-62.
  5. Rochman, Y. et al. (2010) Proc Natl Acad Sci U S A 107, 19455-60.
  6. Brown, V.I. et al. (2007) Cancer Res 67, 9963-70.
Entrez-Gene Id
85480
Swiss-Prot Acc.
Q969D9
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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