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3158
Human Interferon-γ (IFN-γ)
Cytokines
Growth Factors and Cytokines

Human Interferon-γ (IFN-γ) #3158

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# Product Name Application Reactivity
Human Interferon-γ (IFN-γ): Image 1

Western blot analysis of extracts from HeLa cells, untreated or treated with various concentrations of Human Interferon-γ, using Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb #9167 (upper) or with total Stat1 Antibody #9172 (lower).

Product Usage Information

Working concentration generally ranges from 10-50 ng/ml.

Storage

Lyophilized product is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration ranging from 0.1 to 1 mg/ml which can be further diluted in aqueous solutions as needed. It is recommended to add carrier protein (0.1% HSA or BSA) for long term storage.

Product Description

Recombinant human IFN-γ was expressed in E. coli and lyophilized in 10mM Sodium Phosphate buffer pH 7.4. Purity was greater than 95% as determined by RP-HPLC and SDS-PAGE.

Purity

>95%

Source / Purification

Recombinant human IFN-γ expressed in E. coli

Background

Interferons (IFNs) appear both locally and systematically early after viral infection and participate in limiting the spread of infection. They also affect cell differentiation, growth, surface antigen expression and immunoregulation (1). There are three naturally occurring interferons: α, β and γ. IFN-α is derived from lymphoblastic tissue and has a number of therapeutic applications in the treatment of various human cancers and diseases of viral origin. Recombinant IFN-α from both natural and synthetic genes binds to a common cell surface receptor and induces antiviral activity in a variety of cell lines. When binding to discrete cell surface receptors on target cells, IFN-α induces rapid changes in Jak/Stat phosphorylation, which initiates the Jak/Stat signaling pathway (2). IFN-α signaling also involves production of DAG without an increased intracellular free calcium concentration and the subsequent activation of calcium-independent isoforms of PKC (β and ε) (3). All IFN-α signaling pathways lead to final alterations of gene expression, which mediate their pleiotropic biologic activities.

IFN-γ, also known as type II interferon, is produced mainly in activated T lymphocytes and natural killer cells (4) and has broad effects on various cells of the immune system. Synthesis of IFN-γ is induced by many signaling proteins including IL-2, FGF, and EGF.

  1. Stiehm, E.R. et al. (1982) Ann Intern Med 96, 80-93.
  2. Pellegrini, S. et al. (1989) Mol Cell Biol 9, 4605-12.
  3. Pfeffer, L.M. and Colamonici, O.R. (1991) Pharmacol Ther 52, 149-57.
  4. Young, H.A. and Hardy, K.J. (1995) J Leukoc Biol 58, 373-81.

Pathways & Proteins

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Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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