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8900
Human Interleukin-1β (hIL-1β)
Cytokines
Growth Factors and Cytokines

Human Interleukin-1β (hIL-1β) #8900

Reviews ()
Citations (3)
Human Interleukin-1β (hIL-1β): Image 1

The production of IL-8 by primary human fibroblasts cultured with increasing concentrations of human IL-1β was assessed. Media from cells incubated with IL-1β for 24 hours was collected and assayed for IL-8 by ELISA and the OD450-OD650 was determined.

Human Interleukin-1β (hIL-1β): Image 2

The purity of recombinant hIL-1β was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hIL-1β and staining overnight with Coomassie Blue.

Human Interleukin-1β (hIL-1β): Image 3

Western blot analysis of extracts from MCF-7 cells, untreated or treated with hIL-1β for 20 minutes, using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033 (upper) and NF-κB p65 Antibody #3034 (lower).

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hIL-1β. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Storage

Stable in lyophilized state at -20°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

Product Description

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-1β. All lots are greater than 98% pure.

Molecular Formula

Recombinant hIL-1β does not have a Met on the amino terminus and has a calculated MW of 17,377. DTT-reduced and non-reduced protein migrate as 18 kDa polypeptides. The expected amino-terminal APVRS of recombinant hIL-1β was verified by amino acid sequencing.

Source / Purification

Recombinant human IL-1β (hIL-1β) Ala117-Ser269 (Accession #NM_000576) was produced in E. coli at Cell Signaling Technology.

Background

IL-1β is a pro-inflammatory cytokine produced predominantly by activated monocytes and epithelial cells (1). Precursor IL-1β is cleaved by caspase-1 and mature IL-1β is then secreted (1-3). Target cells include macrophages and many other cell types. Signaling by IL-1β involves IL-1β binding to IL-1 accessory protein (IL-1-AcP) and then the complex binds to IL-1RI (1,2). Signaling is through activation of MAP kinase and NF-κB pathways (1,2). IL-1β also binds to IL-1RII that lacks an intracellular signaling domain and thereby serves as a high affinity decoy receptor. IL-1β binding to IL-1RI is inhibited by the negative regulator, IL-1R antagonist (IL-1Ra). IL-1Ra binding to IL-1RI does not signal and serves to block IL-1β signaling. IL-1β plays critical roles in the acute phase response and sepsis (1-3).

  1. Dinarello, C.A. (1996) Blood 87, 2095-147.
  2. Allan, S.M. et al. (2005) Nat Rev Immunol 5, 629-40.
  3. Kramer, F. et al. (2008) Mol Immunol 45, 2678-89.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
To Purchase # 8900SC
Product # Size Price
8900SC
10 µg  (With Carrier) $ 221
8900SF
10 µg  (Carrier Free) $ 221