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8931
Human Interleukin-22 (hIL-22)

Human Interleukin-22 (hIL-22) #8931

Coomassie Gel Image 1

The purity of recombinant hIL-22 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hIL-22 and staining overnight with Coomassie Blue.

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Bioactivity Image 2

The production of IL-10 by COLO 205 cells cultured with increasing concentrations of hIL-22 was assessed. Media from the cells incubated with hIL-22 for 48 hours was collected and assayed for IL-10 by ELISA and the OD450-OD650 was determined.

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Western Blotting Image 3

Western blot analysis of extracts from COLO 205 cells, untreated or treated with hIL-22 for 15 minutes, using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145 (upper) or Stat3 Antibody #9132 (lower).

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Recombinant human IL-22 (hIL-22) Ala34-Ile179 (Accession #NP_065386) was expressed in human 293 cells at Cell Signaling Technology.

Purity:

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-22. All lots are greater than 98% pure.

Molecular Formula:

Recombinant hIL-22 contains no "tags" and the nonglycosylated protein has a calculated MW of 16,749. DTT-reduced and non-reduced protein migrate as 34 kDa polypeptides. Lower mobility and heterogeneity in SDS PAGE are due to glycosylation. The expected amino-terminal APISS of recombinant hIL-22 was verified by amino acid sequencing.

Bioactivity:

The bioactivity of recombinant IL-22 was determined by its ability to induce IL-10 production by COLO 205 cells. The ED50 of each lot is between 100-300 pg/ml.

Endotoxin:

Less than 0.01 ng endotoxin/1μg hIL-22.

Formulation:

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hIL-22. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Storage:

Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

IL-22, a member of the IL-10 family (1,2), is expressed by Th17 CD4+ T cells, activated T cells, Th1 cells and NK cells (3). IL-22 induces proinflammatory responses, drives production of antimicrobial peptides, and is involved in tissue-repair and wound-healing responses (1). The IL-22 receptor is a heterodimer of IL-22R1 and IL-10R2 (4). Expression of IL-22R is restricted to tissue-resident cells, particularly those of epithelial origin, whereas the IL-10R2 chain is expressed in many more cell types. IL-22 induces phosphorylation of Jak1 and Tyk2, leading to activation of Stat3 and, to a lesser extent, Stat1 and Stat5 (1). IL-22 responses can involve activation of the MEK-ERK-RSK, JNK-SAPK, and p38 pathways (1). Elevated levels of IL-22 have been associated with Crohn's disease and rheumatoid arthritis. IL-22 plays an essential role in host response to the pulmonary pathogen Klebsiella pneumoniae (5).

  1. Ouyang, W. et al. (2008) Immunity 28, 454-67.
  2. Dumoutier, L. et al. (2000) Proc Natl Acad Sci U S A 97, 10144-9.
  3. Takatori, H. et al. (2008) Mod Rheumatol 18, 533-41.
  4. Nagalakshmi, M.L. et al. (2004) Int Immunopharmacol 4, 679-91.
  5. Aujla, S.J. et al. (2008) Nat Med 14, 275-81.
Entrez-Gene Id
50616
Swiss-Prot Acc.
Q9GZX6
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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To Purchase # 8931SC

Product Number Size Price
8931SC 10 µg (With Carrier) $204.00.0
8931SF 10 µg (Carrier Free) $204.00.0
Quantity Subtotal
$0.00