View Featured Offers >>
5164
Human Interleukin-28A (hIL-28A/IFN-λ2)
Cytokines & Growth Factors
Growth Factors and Cytokines

Human Interleukin-28A (hIL-28A/IFN-λ2) #5164

Citations (1)
The bioactivity of hIL-28A was determined in a virus protection assay. Hep G2 cells were pre-treated with increasing concentrations of hIL-28A for 24 hours. Cells were then inoculated with encephalomyocarditis virus (EMCV) and incubated for an additional 48 hours. Surviving cells were fixed and stained with crystal violet and the OD595 was determined.
The purity of recombinant hIL-28A was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hIL-28A and staining overnight with Coomassie Blue.
Western blot analysis of extracts from Hep G2 cells, untreated or treated with hIL-28A for 30 minutes, using Phospho-Stat3 (Tyr705) (D3A7) Rabbit mAb #9145 (upper) and Stat3 Antibody #9132 (lower).

Storage

Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.
Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

Product Description

MW (kDa) 21
Purity >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-28A. All lots are greater than 98% pure.
Endotoxin Less than 0.01 ng endotoxin/1 μg hIL-28A.
Activity The bioactivity of recombinant hIL-28A was determined in a virus protection assay. The ED50 of each lot is between 10-60 ng/ml.
Molecular Formula Based on amino acid sequencing, greater than 60% of recombinant hIL-28A starts at Val26 (VPVAR) and has a calculated MW of 19,754. The remainder starts at Pro27 (PVARL) or Val28 (VARLH). DTT-reduced and non-reduced protein migrate as 21 kDa polypeptides.

Source / Purification

Recombinant human IL-28A (hIL-28A) Val26-Val200 (Accession #NP_742150) was expressed in human 293 cells at Cell Signaling Technology.

Background

IL-28A is a member of the Interferon λ family of cytokines, which includes IL-29 and IL-28B (1). IL-28A is produced by a number of cell types and shares many functions with Type I Interferons (1-3). IL-28A exhibits anti-viral activities in vitro and in vivo (1,2). IL-28A directly inhibits tumor cell proliferation and promotes anti-tumor immune responses in vivo (1,3). The IL-28 receptor is a heterodimer of the IL28-Rα and IL10-RI (1). IL-28A activates Stat1, Stat3, and Stat5 (1). IL-28Rα expression is limited to a few cell types, including plasmacytoid DC and epithelial cells (1).

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.
Inquiry Info.# 5164