With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hIL-3. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.
Stable in lyophilized state at -20°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles. Maintain sterility. Storage at -20°C should be in a manual defrost freezer.
|Purity||>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-3. All lots are greater than 98% pure.|
|Endotoxin||Less than 0.01 ng endotoxin/1 μg hIL-3.|
|Activity||The bioactivity of recombinant hIL-3 was determined in a TF-1 cell proliferation assay. The ED50 of each lot is between 50 -400 pg/ml.|
|Molecular Formula||Recombinant hIL-3 contains no "tags" and the nonglycosylated protein has a calculated MW of 15,091. DTT-reduced and non-reduced protein migrate as 26 kDa polypeptides. Lower mobility and heterogeneity in SDS PAGE are due to glycosylation. The expected amino-terminal APMTQ of recombinant hIL-3 was verified by amino acid sequencing.|
Recombinant human interleukin-3 (hIL-3) Ala20-Phe152 (Accession # NP_000579) was expressed in human 293 cells at Cell Signaling Technology.
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