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12786
Human Interleukin-6 Receptor α (hIL-6Rα)

Human Interleukin-6 Receptor α (hIL-6Rα) #12786

Coomassie Gel Image 1

The purity of recombinant hIL-6Rα was determined by SDS-PAGE of 6 µg reduced (+) and nonreduced (-) recombinant IL-6Rα and staining overnight with Coomassie Blue.

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Bioactivity Image 2

The ability of hIL-6Rα to enhance IL-6 mediated inhibition of M1 cell proliferation was assessed. M1 Cells were treated with increasing concentrations of hIL-6Rα in the presence of 20 ng/ml Human Interleukin-6 (hIL-6) #8904. After 72 hours, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.

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Recombinant Human Interleukin-6 Receptor α (hIL-6Rα) Leu20 - Asp358 (Accession #NP_000556) was expressed in human 293 cells at Cell Signaling Technology.

Purity:

>90% as determined by SDS-PAGE of 6 μg reduced (+) and nonreduced (-) recombinant hIL-6Rα. All lots are greater than 90% pure.

Molecular Formula:

Molecular Characterization: Recombinant hIL-6Rα has a calculated MW of 37,871. DTT-reduced and non-reduced protein migrate as 66 kDa polypeptides. Lower mobility and heterogeneity in SDS-PAGE are due to glycosylation. The expected amino-terminal LAPRR of recombinant hIL-6Rα was verified by amino acid sequencing.

Bioactivity:

The activity of hIL-6Rα was determined by assessing its ability to enhance IL-6 mediated inhibition of M1 cells proliferation. The ED50 of each lot is between 2 and 15 ng/ml.

Endotoxin:

Less than 0.01 ng endotoxin/1 μg hIL-6Rα.

Formulation:

With carrier: Lyophilized from a 0.22 μm filtered solution of hIL-6Rα in 20 mM Tris, pH 7.2 containing 20 μg BSA per 1 μg

hIL-6Ra. Carrier free: Lyophilized from a 0.22 μm filtered solution of hIL-6Rα in 20 mM Tris, pH 7.2.

Storage:

Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles. Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

The IL-6 receptor is a heterodimeric complex that consists of a ligand-binding IL-6 receptor α (IL-6Rα) subunit and a signaling component, gp130 (1). Binding of IL-6 to IL-6Rα results in dimerization of receptor with gp130 and subsequent STAT3 activation (1). IL-6Rα is cleaved from the cell surface by ADAM17 (1,2). In humans, soluble IL-6Rα is also generated via alternatively spliced mRNA (1,3). Soluble IL-6Rα binds to IL-6 and can stimulate signaling via membrane bound gp130 in a process known as “trans-signaling” (1). It is through trans-signaling that IL-6 stimulates cells that do not express membrane bound IL-6Rα (1).

  1. Rose-John, S. (2012) Int J Biol Sci 8, 1237-47.
  2. Müllberg, J. et al. (1993) Eur J Immunol 23, 473-80.
  3. Lust, J.A. et al. (1992) Cytokine 4, 96-100.
Entrez-Gene Id
3570
Swiss-Prot Acc.
P08887
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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To Purchase # 12786SC

Product Number Size Price
12786SC 10 µg (With Carrier) $204.00.0
12786SF 10 µg (Carrier Free) $204.00.0
Quantity Subtotal
$0.00