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8911
Human Leukemia Inhibitory Factor (hLIF)
Cytokines
Growth Factors and Cytokines

Human Leukemia Inhibitory Factor (hLIF) #8911

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Human Leukemia Inhibitory Factor (hLIF): Image 1

The proliferation of TF-1 cells treated with increasing concentrations of hLIF was assessed. After 72 hr treatment with hLIF, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.

Human Leukemia Inhibitory Factor (hLIF): Image 2

The purity of recombinant hLIF was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hLIF and staining overnight with Coomassie Blue.

Human Leukemia Inhibitory Factor (hLIF): Image 3

Western blot analysis of extracts from TF-1 cells untreated or treated with hLIF for 10 min, using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145 (upper) or Stat3 Antibody #9132 (lower).

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hLIF. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Storage

Stable in lyophilized state at -20°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

Product Description

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hLIF. All lots are greater than 98% pure.

Molecular Formula

Based on amino acid sequencing, greater than 30% of recombinant hLIF has a Met on the amino-terminal Ser23 (MSPLP) and has a calculated MW of 19,847. The remainder starts at Ser23 (SPLPI), Pro24 (PLPIT), or Pro26 (PITPV). DTT-reduced protein migrates as a 20 kDa polypeptide and non-reduced protein migrates as a 17 kDa polypeptide due to intramolecular cystines.

Source / Purification

Recombinant human LIF (hLIF) Ser23-Phe202 (Accession #P15018) was produced in E. coli at Cell Signaling Technology.

Background

Leukemia Inhibitory Factor (LIF) is a 20 kDa pleiotrophic factor belonging to the IL-6 superfamily of cytokines (1). LIF is expressed in a number of tissues and cell types. The LIF receptor is a heterodimer comprised of LIF-R (gp190) and gp130, a common signal transducer for IL-6-type cytokines (1). Depending on cell type and context, LIF/LIF-R can activate Erk, PI3K, and Jak1/Stat1/3 pathways (1,2). LIF has a diverse array of biological activities. Murine embryonic stem cells are dependent on LIF for pluripotency and self-renewal in vitro (1). Exercise- induced LIF secretion in muscle induces myoblast proliferation, suggesting that LIF may play a role in exercise-induced muscle hypertrophy (2). LIF also negatively regulates Th2 and Th17 cell differentiation (3,4).

  1. Mathieu, M.E. et al. (2012) Stem Cell Rev 8, 1-15.
  2. Broholm, C. and Pedersen, B.K. (2010) Exerc Immunol Rev 16, 77-85.
  3. Cao, W. et al. (2011) Immunity 35, 273-84.
  4. Ullah, U. et al. (2012) Sci Rep 2, 464.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
To Purchase # 8911SC
Product # Size Price
8911SC
10 µg  (With Carrier) $ 221
8911SF
10 µg  (Carrier Free) $ 221