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12900
Human MCP-1
Cytokines & Growth Factors
Growth Factors and Cytokines

Human MCP-1 #12900

Citations (0)
hMCP-1-induced migration of THP-1 cells was assessed. THP-1 cells were incubated in a 96-well transwell plate with increasing concentrations of hMCP-1 in the bottom chamber. After 2 hr, the number of THP-1 cells that migrated to the bottom chamber of the transwell was quantified by measuring DNA content using a fluorescent dye.
The purity of recombinant hMCP-1 was determined by SDS-PAGE of 6 µg reduced (+) and nonreduced (-) recombinant hMCP-1 and staining overnight with Coomassie Blue.

Storage

Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles. Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

Product Description

MW (kDa) 8.6
Purity >95% as determined by SDS-PAGE of 6 μg reduced (+) and nonreduced (-) recombinant hMCP-1. All lots are greater than 95% pure.
Endotoxin Less than 0.01 ng endotoxin/1 μg hMCP-1.
Activity The activity of hMCP-1 was determined using a THP-1 cell migration assay. The ED50 of each lot is between 1-11 ng/ml.
Molecular Formula Molecular Characterization: Recombinant hMCP-1 has a calculated MW of 8,685. DTT-reduced protein migrates as a 10 kDa polypeptide. The nonreduced protein migrates at 12 kDa. The expected amino-terminal QPDAI of recombinant hMCP-1 was verified by amino acid sequencing.

Source / Purification

Recombinant Human MCP-1 (hMCP-1) Gln24-Thr99 (Accession #NP_002973) was expressed in E.coli at Cell Signaling Technology.

Background

MCP-1 (CCL2) is the first member of the C-C family of chemokines to be identified (1). C-C chemokines are characterized by two adjacent cysteine residues within the polypeptide, which form an intra-molecular disulfide bond. MCP-1 is a potent chemotactic factor for monocytes/macrophages, T cells and a subset of NK cells (1-4). The MCP-1 receptor, CCR2, is expressed as two splice isoforms, CCR2A and CCR2B, of which CCR2B is the predominant form (1). MCP-1 is secreted by adipocytes and appears to be one of many links between obesity, inflammation, and diabetes (1). MCP-1/CCR2 signaling appears to play a key role in γδ effector T cells recruitment and anti-tumor responses in a murine B16 melanoma model (2). Conversely, CCL2 expression is upregulated in many types of cancer and has been implicated in promoting tumor cell survival, proliferation, and tumor associated inflammation (4).

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.
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