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27313
Human/Mouse/Rat Activin A Recombinant Protein
Cytokines
Growth Factors and Cytokines

Human/Mouse/Rat Activin A Recombinant Protein #27313

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Human/Mouse/Rat Activin A Recombinant Protein: Image 1
Serial dilutions of Human/Mouse/Rat Activin A Recombinant Protein were added to MPC-11 cells. Cell cytotoxicity was measured and the linear portion of the curve was used to calculate the ED50.
Human/Mouse/Rat Activin A Recombinant Protein: Image 2
The purity of Human/Mouse/Rat Activin A Recombinant Protein was determined by SDS-PAGE of 1 µg reduced (+) and non-reduced (-) recombinant h/m/r Activin A and staining with Coomassie Blue. h/m/r Activin A is a homodimer with a predicted total molecular weight (MW) of 26.2 kDa with each subunit equaling 13.1 kDa.

Storage

Human/Mouse/Rat Activin A Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage

Product Description

MW (kDa) 26
Purity A greater than or equal to 95% purity was determined by SDS-PAGE.
Endotoxin Endotoxin levels are less than or equal to 1 EU / 1 μg h/m/r Activin A.
Activity The bioactivity of recombinant h/m/r Activin A was determined in an MPC-11 cell cytotoxicity assay. The ED50 of each lot is less than or equal to 10 ng/ml.

Source / Purification

Recombinant human/mouse/rat Activin A was expressed in E. coli and is supplied in a lyophilized form.

Background

Activin A is a pleiotropic cytokine that plays an important role in the regulation of the immune response and has been shown to possess both proinflammatory and anti-inflammatory activities (1-3). This cytokine is a member of the TGF-β superfamily known to signal through the Smad- and MAPK-dependent pathways by binding to the activin receptors type I (Alk2, Alk4) and type II (ActRIIA, ActRIIB) (1,2). Activin A is expressed by several cell types, including endothelial cells, mast cells, monocytes, macrophages, and dendritic cells (DCs) (4-6). The effect that Activin A has on the immune response makes it an important cytokine to study when looking at different diseases, such as allergy, autoimmunity, acute and chronic inflammation, and cancer (1,5).
  1. Morianos, I. et al. (2019) J Autoimmun 104, 102314.
  2. Seeger, P. et al. (2014) J Immunol 192, 1241-8.
  3. Samitas, K. et al. (2016) Eur Respir J 47, 769-82.
  4. Sierra-Filardi, E. et al. (2011) Blood 117, 5092-101.
  5. Hedger, M.P. et al. (2011) Vitam Horm 85, 255-97.
  6. Sozzani, S. and Musso, T. (2011) Blood 117, 5013-5.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
To Purchase # 27313S
Product # Size Price
27313S
10 µg $ 207