View Featured Offers >>
5592
Human Neurotrophin-4 (hNT-4)
Cytokines & Growth Factors
Growth Factors and Cytokines

Human Neurotrophin-4 (hNT-4) #5592

Citations (0)
The activity of hNT-4 was assessed. After starvation, TrkB transfected NIH/3T3 cells were treated with increasing concentrations of hNT-4 for 5 minutes. Cells were lysed, and phospho-TrkB was quatified using PathScan® Phospho-TrkB (panTyr) Sandwhich ELISA Kit #7108. OD450 is shown.
The purity of recombinant hNT-4 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hNT-4 and staining overnight with Coomassie Blue.
Western blot analysis of extracts from TrkB transfected NIH/3T3 cells untreated and treated with increasing concentrations of hNT-4 for 5 minutes, using Phospho-TrkA (Tyr674/675)/TrkB (Tyr706/707) (C50F3) Rabbit mAb #4621 (upper) or TrkB (80E3) Rabbit mAb #4603 (lower).

Storage

Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.
Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

Product Description

MW (kDa) 14
Purity >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hNT-4. All lots are greater than 98% pure.
Endotoxin Less than 0.01 ng endotoxin/1 μg hNT-4.
Activity The activity of hNT-4 was assessed by quatification of phospho-TrkB in TrkB transfected NIH/3T3 cells. The observed ED50 values are between 1-4 ng/ml.
Molecular Formula Recombinant hNT-4 does not have a Met on the amino terminus and has a calculated MW of 13,923. DTT-reduced and non-reduced protein migrate as 14 kDa polypeptides. The expected amino-terminal GVSET of recombinant hNT-4 was verified by amino acid sequencing.

Source / Purification

Recombinant human Neurotrophin-4 (hNT-4) Gly81-Ala210 (Accession #NP_006170) was produced in E.coli at Cell Signaling Technology.

Background

NT-4 is a member of the structurally related neurotrophin family of proteins, which includes β-NGF, BDNF and NT-3 (1). NT-4 is expressed in a number of cell types and tissues, including neuronal cells, normal breast epithelial cells, melanocytes, activated T cells, and granulocytes (1-5). NT-4 is required for the development of peripheral sensory neurons (6,7). NT-4 may be important for the development of long term memory (8). Increased NT-4 expression in melanoma cells promotes cell proliferation and migration (5). NT-4 is secreted from cells as a precursor protein, which is proteolytically cleaved into the mature form (1). NT-4 signaling is mediated through two distinct receptors, the neurotrophin receptor p75NTR and the Trk tyrosine kinase receptor TrkB. While all neurotrophins bind to the p75NTR receptor, NT-4 preferentially binds to the TrkB receptor (1).

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.
Inquiry Info.# 5592