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8925
Human Stem Cell Factor (hSCF)
Cytokines
Growth Factors and Cytokines

Human Stem Cell Factor (hSCF) #8925

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other Image 1 - Human Stem Cell Factor (hSCF)

The proliferation of M-07e cells treated with increasing concentrations of hSCF was assessed. After 48 hour treatment with hSCF, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.

other Image 2 - Human Stem Cell Factor (hSCF)

The purity of recombinant hSCF was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hSCF and staining overnight with Coomassie Blue.

other Image 3 - Human Stem Cell Factor (hSCF)

Western blot analysis of extracts from M-07e cells untreated or treated with hSCF for 5 minutes, using Phospho-c-Kit (Tyr719) Antibody #3391 (upper) and c-Kit Antibody #3392 (lower)

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hSCF. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Storage

Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

Product Description

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hSCF. All lots are greater than 98% pure.

Molecular Formula

Recombinant hSCF contains no "tags" and the nonglycosylated protein has a calculated MW of 18,458. DTT-reduced and non-reduced protein migrate as 22-35 kDa polypeptides. Lower mobility and heterogeneity in SDS-PAGE are due to glycosylation. The expected amino-terminal EGICR of recombinant hSCF was verified by amino acid sequencing.

Source / Purification

Recombinant human SCF (hSCF) Glu26-Ala189 (Accession #P21583) was expressed in human 293 cells at Cell Signaling Technology.

Background

SCF is produced by endothelial cells, fibroblasts, keratinocytes, gut epithelial cells and tumor cells (1,2). SCF is critical for hematopoiesis and mast cell differentiation and has additional roles in survival and function of other cell types (1). Some tumor cell proliferation and invasiveness are promoted by SCF (3). Tumor-derived SCF appears to be involved in expansion of myeloid-derived suppressor cells that in-turn limits proliferation of tumor-infiltrating T-cells (4). SCF may have additional roles in the tumor microenvironment (2). SCF is either soluble or integral membrane and the form is dependant on variation in splicing or proteolytic release (1). SCF binds to the receptor tyrosine kinase c-kit and induces activation of the AKT, ERK, JNK and p38 pathways (5,6).

  1. Broudy, V.C. (1997) Blood 90, 1345-64.
  2. Huang, B. et al. (2008) Blood 112, 1269-79.
  3. Yasuda, A. et al. (2006) Mol Cancer 5, 46.
  4. Pan, P.Y. et al. (2008) Blood 111, 219-28.
  5. Samayawardhena, L.A. and Pallen, C.J. (2008) J Biol Chem 283, 29175-85.
  6. Huang, H.M. et al. (2000) Blood 96, 1764-71.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
To Purchase # 8925SC
Product # Size Price
8925SC
10 µg  (With Carrier) $ 221
8925SF
10 µg  (Carrier Free) $ 221