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11946
Human TL1A/TNFSF15 (hTL1A)
Cytokines & Growth Factors
Growth Factors and Cytokines

Human TL1A/TNFSF15 (hTL1A) #11946

Citations (0)
The viability of TF-1 cells treated with increasing amounts of hTL1A in the presence of 10 µg/ml cyclohexamide was determined. After a 24 hr treatment with hTL1A, cells were incubated with tetrazolium salt and the OD450 - OD650 was determined.
The purity of recombinant hTL1A was determined by SDS-PAGE of 6 µg reduced (+) and nonreduced (-) recombinant hTL1A and staining overnight with Coomassie Blue.

Storage

Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles. Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

Product Description

MW (kDa) 21
Purity >95% as determined by SDS-PAGE of 6 μg reduced (+) and nonreduced (-) recombinant hTL1A. All lots are greater than 95% pure.
Endotoxin Less than 0.01 ng endotoxin/1 μg hTL1A.
Activity The bioactivity of hTL1A was determined in a TF-1 cell viability assay. The ED50 of each lot is between 2-15 ng/ml.
Molecular Formula Recombinant hTL1A has a calculated MW of 20,0473. DTT-reduced protein migrates as a 21 kDa polypeptide. The nonreduced protein migrates as a 21 kDa monomer and 38 kDa cystine-linked homodimer. The expected amino terminus of recombinant hTL1A was verified by amino acid sequencing.

Source / Purification

Recombinant Human TL1A Leu72-Leu251 (Accession #NP_095150) was expressed in E.coli at Cell Signaling Technology.

Background

TL1A (TNFSF15), a member of the TNF superfamily of proteins, is a splice variant of the TL1/VEGI gene (1). Endothelial cells, monocytes, macrophages, and dendritic cells express TL1A, which is upregulated by proinflammatory cytokines, microorganisms, and AMPK activation (1-4). TL1A activates the NF-κB and JNK pathways through its receptor, DR3 (1,5). TL1A may function as a costimulatory signal for T cell activation, specifically regulating Th17 cell development and proliferation (1,2,6). Mouse models suggest a role for TL1A as a driver for the inflammation and pathogenesis associated with inflammatory bowel disease (7,8).

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.
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