The proliferation of HUVEC treated with increasing concentrations of hVEGF121 was assessed. After 72-hour treatment with hVEGF121 cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.
The purity of recombinant hVEGF121 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hVEGF121 and staining overnight with Coomassie Blue.
Western blot analysis of extracts from HUVEC untreated or treated with hVEGF121 for 15 minutes, using Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb #9215 (upper) and p38 MAPK Antibody #9212 (lower).
With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hVEGF121. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.
Stable in lyophilized state at -20°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.Maintain sterility. Storage at -20°C should be in a manual defrost freezer.
>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hVEGF121. All lots are greater than 98% pure.
Less than 0.01 ng endotoxin/1μg hVEGF121.
The bioactivity of recombinant hVEGF121 was determined in a cell proliferation assay using HUVEC. The ED50 of each lot is between 0.5-2 ng/ml.
Recombinant hVEGF121 contains no "tags" and the nonglycosylated protein has a calculated MW of 14,057. DTT-reduced protein migrates as a 14-22 kDa polypeptide. Heterogeneity in SDS-PAGE is due to glycosylation. The non-reduced cystine-linked homodimer migrates as a 30-36 kDa protein. The expected amino-terminal APMAE of recombinant hVEGF121 was verified by amino acid sequencing.
Recombinant human VEGF121 (hVEGF121) Ala207-Arg327 (Accession #NP_001020541.2) was expressed in human 293 cells at Cell Signaling Technology.
VEGF121 is the second most abundant splice variant of VEGF-A (1,2). VEGF121 is produced by endothelial cells, macrophages, T-cells and other cell types. VEGF121 is involved in angiogenesis, vascular endothelial cell survival, growth, migration and vascular permeability (1). VEGF121 expression is induced by hypoxia, inflammatory cytokines and through oncogene products in tumors (1-3). VEGF121 binds to VEGFR1 and VEGFR2 receptor tyrosine kinases (1). Binding of VEGF121 to VEGFR1 and VEGFR2 leads to activation of pathways involving PI3K/AKT, P38 MAPK, and FAK (1). VEGF plays a key role in tumor angiogenesis in many cancers (2).
Explore pathways + proteins related to this product.
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.