|8904||Human Interleukin-6 (hIL-6)||
Western blot analysis of extracts from human myeloma cells stimulated with rhIL-6 (100 ng/ml for 10 min.), using Phospho-Stat1(Tyr701) Antibody #9171 (upper A), Stat1 Antibody #9172 (lower A), Phospho-Stat3(Tyr705) Antibody #9131 (upper B), Stat3 Antibody #9132 (lower B), Phospho-Jak1(Tyr1022/1023) Antibody #3331 (upper C) and Jak1 Antibody #3332 (lower C).
CST recommends using 50-100 ng/ml for stimulation of IL-6 signaling.
Human IL-6 is supplied in PBS buffer containing 0.1% BSA. Aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. F
Human IL-6 cDNA was subcloned into a prokaryotic expression vector, expressed in E. coli, and recombinant human Interleukin-6 (rhIL-6) protein was purified.
Human Recombinant Protein
Interleukin-6 (IL-6) is a pleiotropic cytokine with central roles in immune regulation, inflammation, hematopoiesis, and oncogenesis. Its biological activities are shared by other IL-6-family members such as leukemia inhibitory factor and oncostatin M (1). To exert its biological effects, IL-6 must bind to the IL-6 receptor (IL-6R), composed of two alpha chains (IL-6Ralpha , 80 kDa) and two beta chains (IL-6Rbeta or gp130, 130 kDa). Two moieties of IL-6 and two pairs of these receptor chains form a functional hexameric IL-6R complex. The subsequent intracellular signaling events are activated via gp130, which is the common signal transducer of cytokines related to IL-6. Activation of the IL-6R stimulates at least three major signaling pathways : JAK/STAT, PI-3 kinase and MAPK signaling pathways (2,3).
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